高效液相色谱法测定大鼠血清染料木素浓度  

Determination of Genistein in Rat Serum by HPLC

作　　者：任国峰[1] 汤凌[1] 谭鸿毅[2] 杨爱青[1] 阳国平[2] 黄忆明[1] 

机构地区：[1]中南大学公共卫生学院,湖南长沙410078 [2]中南大学湘雅三医院,湖南长沙410013

出　　处：《生物技术》2009年第4期51-53,共3页Biotechnology

基　　金：国家自然科学基金面上项目("大豆异黄酮抗良性前列腺增生的实验研究";30500407)资助

摘　　要：目的：建立大鼠血清中染料木素浓度的HPLC测定方法。方法：大鼠血清以叔丁基甲醚萃取,萃取物用氮气吹干后,用甲醇溶解用于色谱分析。色谱条件：采用Thermo C18柱（250mm×4.6mm,5μm）;以乙腈-0.02mol/L磷酸二氢钾（35∶65,pH=4.3）为流动相;流速为1.0mL/min;检测波长为260nm;柱温为40℃;进样量为10μL。结果：染料木素最低检测浓度为0.01mg/L;标准曲线线性范围为0.01-10.00μg/mL（r=0.9998）;相对回收率为（101.31±3.47）%;日内RSD与日间RSD均小于10.00%。结论：该方法简便、快速、灵敏度高,重现性及稳定性较好,适用于大鼠血清染料木素浓度测定和药代动力学的研究。Objective：An assay method for genistein in rat serum by high performance liquid chromatography was established. Method： Serum sample was extracted with tert - butyl methyl ether （TBME） for HPLC analysis. The HPLC assay was conducted using Thermo C18 column （4.6mm × 250mm, 5μm） with acetonitrile- 0.02mol/L potassium dihydrogen phosphate （35：65） as mobile phase at a flow- rate of 1.0 mL· min^-1. 10μL of sample solution were injected into the C18 column where the temperature was 40℃. The detection wave length was 260nm. Resuit：The limit of detection for genistein was 0.01 mg·L^-1 in serum sample. The linearity range for genistein was obtained from 0.01 mg· L^-1 to 10. 00mg·L^-1, r = 0.9998. The recoveries of genistein were 100.1% ± 1.8 %. The relative standard deviations of the intra - day and inter - day were less than 10%. Concluslon： The method is simple, rapid, sensitive, and accurate, and has a good reproducibility and stability, which may be used for serum concentration monitoring and pharmacokinetics study for genistein.

关 键 词：高效液相色谱法 染料木素 大鼠 血清浓度 

分 类 号：R927.2[医药卫生—药学]