HLA-DR基因及乙肝病毒基因型与肝细胞癌相关性的初步研究  

Study on the Correlation between HLA-DR Alleles,Hepatitis B Virus Genotype and Hepatocellular Carcinoma

作　　者：王义成[1] 李建忠[1] 李长缨 

机构地区：[1]青岛市传染病医院,山东青岛266033 [2]青岛市中心血站,山东青岛266071

出　　处：《预防医学论坛》2009年第9期818-819,822,共3页Preventive Medicine Tribune

摘　　要：[目的]探讨乙型肝炎病毒（HBV）感染人群HLA—DR基因、HBV基因型与肝细胞癌的相关性。[方法]2007～2008年，应用序列特异性引物-聚合酶链反应（SSP—PCR）方法与荧光PCR（探针）法，分别对40例慢性乙肝患者（试验1组）、38例肝硬化患者（JR验2组）和34例肝细胞癌患者（试验3组）的HLA—DR等位基因、HBV基因型进行检测，并以中华骨髓库山东分库无血缘关系的自愿骨髓捐献者1383例为对照，分析其表达与肝细胞癌的相关性。[结果]HLA—DR1基因的检出率，试验3组为14．71％，对照组为4．85％（P〈0．05）；HLA—DR13基因的检出率，试验3组为14．71％，试验1组为0．00％（P〈O．05）；试验2组与试验3组各等位基因表型的检出率差异均无统计学意义（P〉0．05）。HBV基因型B型、C型、非B非C型所占比例，试验1、2、3组的差异无统计学意义（P〉0．05）。试验3组中，HLA—DR1、DR13阳性的5例均为HBV基因C型；其他29例HBV基因C型23例、非B非C型6例（P〉0．05）。[结论]HLA—DR1可能是肝细胞癌的易感基因．携带HLA—DR13基因位点的HBV感染者更易发展为肝细胞癌。[Objective]To investigate the correlation between HLA-DR alleles, HBV genotype and hepatocellular carcinoma in patients with HBV infection. [Methods]From 2007 to 2008 ,experiment groups were composed of 40 patients with chronic hepatitis B （group1）, 38 patients with liver cirrhosis （group2） and 34 patients with hepatocellular carcinoma （group3）. The control group was composed of 1 383 marrow donors from China Marrow Bank of Shandong Branch. HLADR were typed by PCR-SSP. Hepatitis B virus genotypes were typed by real time PCR（Taqman probe） to analyze the association between HLA-DR alleles, HBV genotype and hepatocellular carcinoma. [Results]The detection rate of HLA-DR1 was 14.71% in group 3 and 4.85% in control group（ P d0.05）;The detection rate of HLA-DR13 was 14.71% in group3 and 0.00% in group 1（ P〈0.05） ;there was no significant difference in the detection rate of HLA-DR between group 2 and group 3（ P 〉0.05）. There was no significant difference in the proportion of HBV genotype B,C and neither B nor C among groupl,group 2 and group 3（ P 〉0.05）. In group 3,the 5 patients who carried HLA-DR1 and HLA-DR13 allelic genes were all HBV genotype C,in the other 29 patients.23 patients were HBV genotype C,6 patients were HBV genotype neither B nor C（ P 〉0.05）. [Conclusion]The HLA-DR 1 might be associated with hepatocellular carcinoma. Petients with HBV infection,who carried HLA-DR13 allelic genes had hepatocellular carcinoma easier.

关 键 词：肝细胞癌 HLA—DR基因 HBV基因型 

分 类 号：R516.2[医药卫生—内科学]