体外受精中精卵孵育时间不同对妊娠结局的影响  

Influence of Incubation of Oocytes and Sperm for Different Period During IVF-ET on Pregnancy Outcome

作　　者：徐岚[1] 徐望明[1] 杨菁[1] 敖良飞[1] 

机构地区：[1]武汉大学人民医院生殖中心,湖北武汉430060

出　　处：《中国误诊学杂志》2009年第9期2022-2024,共3页Chinese Journal of Misdiagnostics

摘　　要：目的：比较体外受精-胚胎移植中不同时间的精卵孵育方法（4～6 h和16～18 h）对妊娠结局的影响,探讨不同时间精卵孵育的机制及临床应用价值。方法：272例体外受精-胚胎移植（IVF-ET）周期根据精卵孵育不同时间分为两组。A组：199例采集的卵子,在加精后受精4～6 h取出放入新鲜培养液中培养,观察受精情况。B组：73例采集的卵子,加精过夜培养后（16～18 h）取出放入同种培养环境中并观察受精情况。比较两组的受精率,卵裂率,优质胚胎率,妊娠率以及种植率。结果：A组受精率和优质胚胎率明显高于B组,有统计学意义。A组种植率高于B组,但无统计学意义。结论：精卵孵育时间缩短能提高受精率和胚胎的质量,从而提高胚胎移植的种植潜能。Objective：To compare the effection about the incubation of insemination for different time during IVF-ET on the pregnant outcome in human.Method：272 women receving IVF-ET treatment were divided into group A（199 cases） and groupB（73 cases）.In group A,patients oocytes mixed with sperm of their own husband were incubated for 4～6 hours,then the oocytes were washed and tranferred into the fresh medium.In group B,the oocytes were incubated with sperms for 16～18 hours. The rates of fertility ,cleavage ,embryo of high quality, pregnancy and implantation of two groups were compared respectively. Results ：There was significant difference of the fertility rate （81.8 % vs 76.0%） and the rate of embryo in high quality （57.2% vs 50.9%）. There was no statistically significant difference of the cleavage rate,pregnant rate and implantated rate. Conclusion：The short time incubation of oocytes and sperm is a simple and effective way in IVF-ET as it can promote the embryo quality and raise fertility rate.

关 键 词：受精 体外 胚胎移植 时间因素 人类 妊娠 女(雌)性 

分 类 号：R321-33[医药卫生—人体解剖和组织胚胎学]