山东、安徽两省栽培番茄烟粉虱传双生病毒的PCR检测及序列分析  

PCR Detection and Sequence Analysis of Whitefly-transmitted Geminivirus in Tomato from Anhui and Shandong Provinces

作　　者：余文贵[1] 赵统敏[1] 杨玛丽[1] 赵丽萍[1] 季英华[2] 周益军[2] 

机构地区：[1]江苏省农业科学院蔬菜研究所 [2]江苏省农业科学院植物保护研究所

出　　处：《江苏农业学报》2009年第4期747-751,共5页Jiangsu Journal of Agricultural Sciences

基　　金：国家公益性行业科研专项(ny-hyzx-07-007);江苏省科技支撑计划项目(BE2008371);江苏省高技术研究计划项目(BG2007302);江苏省"333"工程(5310713)

摘　　要：2008年秋季山东菏泽、安徽淮北保护地栽培番茄上发生了一种新的病害,对两地采集的4份病样进行了分子检测,结果表明4份样品所感染的病毒均属于菜豆金色花叶病毒属(Begomovirus),同源率在98.7%以上。并对其中代表样品AH1进行了DNA-A克隆和序列分析,结果表明AH1全长2786个核苷酸,共编码6个ORF。基因组比较发现,AH1 DNA-A与浙江省和上海市报道的番茄黄化曲叶病毒(Tomato yellow leaf curl virus,TYLCV)同源性达到99.3%以上。这是该病毒在安徽、山东两省的首次报道,值得关注。A new virus disease occurred in Anhui and Shandong provinces in the autumn of 2008, and four samples were collected from the tomatoes showing leaf curl symptoms. Molecular diagnosis was carried out by PCR and the result showed that the four samples were infected by begomovirus and the 500 bp sequences of them shared over 98.7% identity from each other. The complete nueleotide sequence of the typical samples AH1 DNA-A was determined to be 2 786 nts encoding six potential ORFs. Blast results revealed that it shared over 99. 3% nucleotide sequence identity with tomato yellow leaf curl virus （TYLCV） DNA-A. This is the first report of TYLCV in Anhui and Shandong provinces.

关 键 词：番茄黄化曲叶病(TYLCD) DNA-A 序列分析 分子特征 

分 类 号：Q78[生物学—分子生物学]