栗疫病菌泛素核糖体L40融合蛋白基因的克隆与分析  

Molecular Cloning and Analysis of Ubiquitin and Ribosomal Protein L40 Fusion Protein from Cryphonectria parasitica

作　　者：乔广行[1] 李涛[1,2] 王克荣[1] 

机构地区：[1]南京农业大学农业部病虫监测与治理重点开放实验室,江苏南京210095 [2]浙江松阳大地农资有限公司,浙江丽水323000

出　　处：《江苏农业学报》2009年第4期758-762,共5页Jiangsu Journal of Agricultural Sciences

基　　金：国家自然科学基金项目(30470062)

摘　　要：用酿酒酵母(Saccharyomyces cerevisae)泛素融合蛋白基因从COGEME(Consortium for the functional ge-nomics of microbial eukaryotes)植物病原菌EST(Expressed sequence tag)库中BLAST(Basic local alignment searchtool)得到栗疫病菌的泛素融合蛋白EST,设计引物从栗疫病菌cDNA中克隆到该基因并测序,得到在进化上高度保守的基因,该基因开放阅读框为387 bp,编码128个氨基酸残基组成的泛素融合蛋白前体;由cDNA序列推定的氨基酸序列分析结果表明,泛素融合蛋白前体包括氮末端的泛素结构域(76个氨基酸残基)和碳末端的核糖体蛋白L40结构域(52个氨基酸残基)。该蛋白为高碱性蛋白,碳末端含有一个"锌指"模式结构。与19个物种比较的结果表明,栗疫病菌与真菌泛素融合蛋白氨基酸序列相似度较高,具有高度的保守性。Using ubiquitin fusion protein gene of Saccharyomyces cerevisae as query, BLAST algorithm in the EST pool of COGEME phytopathology was performed and one highly homologous contig which contained the Cryphonectria parasitica ubiquitin fusion protein coding sequence was obtained. With the designed primers, the gene was cloned from the cDNA of C. parasitica and sequenced ,finally conserved cDNA sequence was obtained. The gene has an open reading frame of 387 bp, encoding a 128 amino acid ubiquitin fusion protein precursor composed of a 76 amino acid ubiquitin domain and followed by a 52 amino acid ribosomal protein L40 domain. The deduced precursor protein from the nucleotide sequence is highly basic. The carboxyl terminus of the ubiquitin fusion protein contains the zinc-finger motif. The predicted amino acid sequence of ubiquitin fusion protein is highly conserved when compared to the sequences of homologous proteins from twenty diverse species.

关 键 词：栗疫病菌 泛素融合蛋白 碳端扩展区 核糖体蛋白 

分 类 号：Q78[生物学—分子生物学]