高渗高胶液体对失血性休克兔肿瘤坏死因子α与白细胞介素6和白细胞介素10mRNA表达的影响  

Experimental study of effect of hypertonic-hyperonconic solution on expression of rabbit mRNA of TNF-α, IL-6 and IL-IO after hemorrhagic shock

作　　者：朱红军[1] 杨美菊[2] 陆士奇[3] 

机构地区：[1]河南省商丘市第一人民医院胸外科,476000 [2]河南省商丘市第一人民医院呼吸内科,476000 [3]苏州大学第一附属医院急诊科

出　　处：《中国医药》2009年第10期768-769,共2页China Medicine

摘　　要：目的研究高渗高胶液体对失血性休克兔肿瘤坏死因子（TNF）-α、白细胞介素（IL）击和IL-10 mRNA表达的影响，并探讨其在急性肺损伤防治中的作用。方法新西兰大白兔40只，完全随机分为实验组和对照组，各20只。采用Wigger’s改良法制作失血性休克模型，分别用5ml／kg的高渗氯化钠羟乙基淀粉40溶液、1．5倍失血量的复方氯化钠溶液复苏动物，然后，2组动物回输1／2失血量的血液。休克前、复苏前、复苏后1h．4h分别经股动脉采血，分离外周单核细胞待测循环中TNF-α、IL-6、IL-10mRNA表达；复苏4h抽取标本后，采取气体栓塞法处死动物，再取肺组织待测TNF-α、IL-6、IL-10mRNA的表达。结果实验组复苏后4h循环中TNF-α、IL-6、IL-10mRNA的表达[分别为（27．3±4．3）、（19．7±2．4）、（27．9±5．6）ng／L]明显高于1h的水平[分别为（14．2±2．3）、（7．5±1．3）、（10．5±4．0）ng／L]，实验组肺组织中TNF-α和IL-6mRNA表达明显低于对照组[实验组分别为（29．6±3．5）、（57．0±4．4）ng／L，对照组分别为（61．3±7．3）、（82．8±6．2）ng／L]，IL-10mRNA表达明显高于对照组[实验组为（139．2±27．3）ng／L、对照组为（89．2±28．7）ng／L]，差异有统计学意义（P〈0．05）。结论高渗高胶液体复苏失血性休克兔能有效地抑制肺部促炎因子的合成与表达，上调抗炎细胞因子的合成与表达，恢复细胞因子网络平衡。Objective To study the effect of hypertonic-hyperonconic solution on expression of rabbit mRNA of TNF-α,IL-6 and IL-10 after hemorrhagic shock. Methods Fourty newzealand rabbits were divided into experimental group（ HH40 group）and control group（ LR solution） randomly with 20 rabbits in each group. A model of hemorrhagic shock was established by Wigger＇s revised method. In 60 minutes the animals were resuscitated with HH40 solution （5ml/kg） which was 1.5 times as much as hemorrhagic volume. The two groups were transferred half of hemorrhagic volumes back to their bodies. The peripheral mononuclear cells were isolated to measure the expressions of TNF-α, IL-6 and IL-10 mRNA in circulation blood 1 hour and 4 hours after resuscitation. The animals were killed with gas embolism after blood sample were collected. Lung tissues were taken to measure expressions of TNF-α, IL-6 and IL-10 mRNA. Results The expressions of local TNF-α and IL-6 mRNA in HH40 group were obviously lower than those in LR group, but IL-10 mRNA was higher than that in LR group. In circulation blood the expressions of TNF-α, IL-6 and IL-10 mRNA were significant in 1 h and 4 h, but not significant difference was observed beteen two groups. Conclusion Hypertonie-hyperoneonic solution can inhibit synthesis and expression of proinflammatory factor, up-regulate synthesis and expression of anti-inflammatory factorand restore the cytokine network balance.

关 键 词：失血性休克 高渗高胶液体 肿瘤坏死因子-α 白细胞介素-6 白细胞介素-10 

分 类 号：R605.971[医药卫生—急诊医学]