钠氢交换体1 mRNA在心衰患者心肌组织中的表达  

The related research for expreesion of myocardial sodium-hydrogen exchanger 1 mRNA in heart failure

作　　者：魏玲[1] 石家冲[1] 石云[1] 卢国良[1] 朱姿英[1] 苏瑶[1] 张利[1] 赵文博[1] 罗国军[1] 杨晓华[1] 张筱军[1] 张亚林[1] 

机构地区：[1]成都军区昆明总医院地方干部病房,650032

出　　处：《中国老年保健医学》2009年第4期18-21,共4页Chinese Journal of Geriatric Care

摘　　要：目的观察钠氢交换体1(sodium-hydrogen exchanger1,NHE1)在心力衰竭患者心肌组织中的表达,探讨NHE1在心衰发生和发展中的作用。方法采用实时荧光定量聚合酶链反应(FQ-PCR)检测35例心力衰竭患者心肌组织NHE1 mRNA的表达水平。结果实时荧光定量PCR(FQ-PCR)检测心功能Ⅰ级组、心功能Ⅱ级组、心功能Ⅲ级组心力衰竭患者心肌组织NHE1 mRNA的△Ct值分别为6.29±0.66、5.01±0.60、4.40±0.74。与心功能Ⅰ级组比较,在心功能Ⅱ级组、心功能Ⅲ级组患者心肌组织中NHE1 mRNA表达明显高于心功能Ⅰ级组(P<0.01),同时与心功能Ⅱ级组比较,心功能Ⅲ级组患者心肌组织中NHE1 mRNA水平增高,且差异有统计学意义(P<0.05)。结论心衰患者心肌组织中存在NHE1 mRNA的高水平表达,提示NHE1可能在心衰的发生和发展过程中起着重要作用。Objectives To detect the Expreesion of Myocardial Sodium-Hydrogen Exchanger 1 mRNA in heart failure, and to study the effects of NHE1 in the incidence of heart failure. Methods The Expreesion of Myocardial Sodium-Hydrogen Exchanger 1 mRNA in 35 heart failure patients was detected by using real-time fluorescence quantitative PCR （FQ-PCR）. Results With the real-time fluorescence quantitative PCR method,the ACt value of class Ⅰ group,class Ⅱ group,class Ⅲgroup were 6.29±0.66,5.01±0.60,4.40±0.74,the expresion of Myocardial Sodium-Hydrogen Exchanger 1 mRNA in group of class Ⅱand class Ⅲ were significantly higher than class Ⅰ group （P〈0.01）. At the same time,Comparison with the group of class Ⅱ ,the Expreesion of Myocardial Sodium-Hydrogen Exchanger 1 mRNA in group of class m was higher level,and the difference was statistically significant （P〈0.05）. Conclusion The mRNA Expreesion of gene Sodium-Hydrogen Exchanger 1 （NHE1） was up-regulated in heart failure,that may prompt the occurrence of heart failure in the process of development and plays an important role.

关 键 词：钠氢交换体1 心力衰竭 心肌组织 实时荧光定量聚合酶链反应 

分 类 号：R541.6[医药卫生—心血管疾病]