共刺激分子CD137单克隆抗体对CD4+CD25+T淋巴细胞死亡方式影响的研究  

Study of Different Death Patterns of Huaman Peripheral Blood CD4+CD25+T Lymphocytes Induced by Anti-CD137 mAb

作　　者：尹柯[1] 周文英[2] 邬伟明[1] 黄瑾[1] 

机构地区：[1]中山大学附属第五医院呼吸内科,广东珠海519000 [2]中山大学附属第五医院中心实验室,广东珠海519000

出　　处：《中国误诊学杂志》2009年第7期1513-1516,共4页Chinese Journal of Misdiagnostics

摘　　要：目的：初步观察抗共刺激分子CD137单克隆抗体对分离培养的人外周血CD4＋CD 25＋T淋巴细胞自噬、凋亡、胀亡现象的影响及其表面特征性标志物FOXp3的表达的变化。方法：采用密度梯度离心法及尼龙棉柱法分离健康志愿者外周血T淋巴细胞,磁性细胞分离器（MACS）分离得到CD4＋CD25＋T淋巴细胞,分别利用电镜及流式细胞仪观察、检测各分组（抗CD137单克隆抗体2μg/ml组、IgG 1同型抗体对照组）的细胞的自噬率、凋亡率、胀亡率及FOXp3的表达。结果：M ACS可成功分离CD4＋CD25＋T淋巴细胞,纯度可达80.3%-89.5%;抗CD 137单克隆抗体可促进人外周血CD4＋CD25＋T淋巴细胞自噬率及凋亡率增加;各组间的胀亡率改变及FOXp3表达差异无统计学意义。结论：抗CD137单克隆抗体可促进CD4＋CD25＋T淋巴细胞凋亡和自噬,其作用机制未发现与胀亡显著相关,而且不影响CD4＋CD25＋T细胞中Foxp3的表达。Objective：To observe the autophagy, apoptosis, oncosis rate and their surface expression of FOXp3 in human peripheral blood CD4＋CD25＋T lymphocytes induced by anti-CD137 monoclonal antibody. Methods . Peripheral blood T lymphocyte of healthy adults were separated by Percoll （1. 073g/ml） and harvested by using nylon column. Then, CD4＋CD25＋T lymphocytes were isolated by MACS（magnetic cell sorting）. The cultured cells were divided into different groups （CD137 mAb group 2 μg /ml, IgG1 isotype control group） and at different time points（0h,72 h） , the autophagy, apoptosis, oncosis and FOXp3 rate were analyzed by electron microscope and flow cytometry. Results： The purity of CD4＋CD25＋T lymphocytes seperated by MACS was 80.3 % 89.50%. The autophagy and apoptosis rate of peripheral CD4＋CD25＋ T lymphocytes were increased by CD137 mAb（P〈0. 05）. There was no obvious change of oncosis rate and FOXp3 among different groups （P〉 0. 05）. Conclusion： Anti-CD137 mAb can increase the autophagy and apoptosis rate of peripheral blood CD4＋CD25＋ T lymphocytes, but have no effect on Foxp3 expression, and the mechanism does not involve the oncosis.

关 键 词：抗原 CD 受体 神经生长因子 肿瘤坏死因子 白细胞介素2 CD4 T淋巴细胞 人类 

分 类 号：R392.12[医药卫生—免疫学]