白簕叶总皂苷提取工艺和含量测定研究  

Extracting Technology and Determination of Total Saponins in Leaves of Acanthopanan Trifoliatus

作　　者：高侠[1] 黎云祥[1] 蔡凌云[1,2] 

机构地区：[1]西华师范大学生命科学学院四川省高校环境科学与生物多样性保护重点实验室,四川省南充市师大路1号637002 [2]凯里学院化学系,贵州省凯里市文化北路22号556003

出　　处：《光谱实验室》2009年第4期814-821,共8页Chinese Journal of Spectroscopy Laboratory

基　　金：四川省教育厅重点项目(2006A078);四川省重点学科项目(SZD0420)资助

摘　　要：采用微波辅助法提取白簕叶中的皂苷类化合物,通过正交实验确定了最佳提取工艺。以齐墩果酸为对照,采用紫外分光光度法在500nm处测定白簕叶中的总皂苷。最佳工艺条件:乙醇浓度70%,微波辐射30s,微波功率160W,固液比1∶10,间歇提取2次。按最佳工艺条件提取总皂苷含量为84.36mg/g。回归方程为:y=1.0167C+0.0006,相关系数r=0.9998,精密度和稳定性试验RSD分别为0.033%和0.034%(n≥6),平均加标回收率为99%,RSD=1.76%(n=6)。用齐墩果酸标准品,采用紫外分光光度法,操作简便,结果准确,灵敏度高,重复性好,为白簕叶中总皂苷含量测定的一种切实可行的方法。The total saponins in leaves of A.trifoliatus（L.）Merr was extracted by Microwave-Assisted method under the extraction condition：70% ethanol as solvent,MAE time 30s,applied power 160W,and solid/liquid ratio 1∶10,and intermittent time was 2.The content of total saponins was determined as 84.36mg/g with the regression equation of y=1.0167C＋0.0006,r=0.9998,the RSD =0.034%（n≥6）,the average recovery of saponins was 99%,RSD=1.76%（n=6）.This preparation is the best technological conditions.Total saponins content was 99%, RSD = 1.76% （n = 6）. This preparation is the best technological conditions. Total saponins content was with standard oleanolic acid solution by ultraviolet spectrophotometry, this optimized process was easy to operate with accurate result, high sensitivity and good reproducibility and can be used as a quantitative analysis method for total saponins in leaves of A. trifoliatus.

关 键 词：白簕叶 总皂苷 微波辅助提取 紫外分光光度法 正交实验 

分 类 号：O657.32[理学—分析化学]