白榆的组织培养与叶片再生研究  

Tissue Culture and Plant Regeneration from Leaf of Siberian Elm

作　　者：王静华[1] 侯建生[2] 刘桂林[3] 王艳[3] 刘海波[3] 

机构地区：[1]河北农业大学林学院,河北保定071001 [2]河北政法职业学院园林系,河北石家庄050061 [3]河北农业大学园林与旅游学院,河北保定071001

出　　处：《西北林学院学报》2009年第5期74-77,共4页Journal of Northwest Forestry University

基　　金：河北省交通厅重点攻关项目(Y-050116)

摘　　要：采用河北沧州市的白榆茎段作为外殖体进行组织培养,在附加不同浓度激素的培养基上对其进行培养,建立其组织培养及叶片再生体系。结果表明,白榆最适增殖培养基是MS培养基+6-BA 0.10 mg.L-1+IBA0.005 mg.L-1,叶片再生的最适培养基均为MS+TDZ0.005 mg.L-1+IAA0.005 mg.L-1,最适生根培养基是MS培养基+IBA0.01 mg.L-1。Stems of siberian elm were used as explants, a tissue culture and plant regeneration system was established. The results showed that the optimal differentiation medium for stem was MS＋6-BA 0. 10 mg · L^-1 ＋IBA0. 005 mg · L^-1; the optimal rooting medium was MS ＋IBA0.01 mg · L^-1; MS medium supplemented with 0. 005 mg · L^-1 TDZ and 0. 005 mg · L^-1IAA was the optimal medium condition for leaf regeneration.

关 键 词：白榆 组织培养 叶片再生 

分 类 号：S792.19[农业科学—林木遗传育种]