曙红-中性红荧光能量转移抑制法测定肝素  

Eosine Y-neutral red fluorescence energy transfer inhibition method for the determination of heparin

作　　者：刘保生[1] 王晶[1] 薛春丽[1] 吕云开[1] 

机构地区：[1]河北大学理化分析中心药物化学与分子诊断教育部省部共建重点实验室,保定071002

出　　处：《分析试验室》2009年第9期16-19,共4页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(20075005)项目资助

摘　　要：在pH6．65的Britton-Robinson（B．R）缓冲溶液中，酸性染料曙红（EY）与碱性染料中性红（NR）之间由于静电吸引而能够发生有效的能量转移，使得能量受体NR荧光增强，同时能量给体EY的荧光猝灭。而肝素的加入对该荧光体系间的能量转移产生了明显的抑制作用，随肝素量的增加EY荧光依次增强，由此建立了测定微量肝素的新方法。结果表明，肝素在0．1～2．0mg／L范围内与EY的荧光增强程度呈良好的线性关系。方法检出限0．071mg／L。用于肝素钠注射液中肝素钠效价的测定，6次平行测定相对标准偏差1．4％～3．6％，回收率95．2％～105．3％。In the Britton-Robinson buffer solution of pH 6.65, significant energy transfer could occur between Eosin Y （EY） and Neutral red （NR） due to static attraction. The fluorescence intensity of EY decreased and that of NR enhanced at the same time. When heparin was added into this system, the fluorescence energy transfer between EY-NR pair was remarkably inhibited and the fluorescence intensity of EY increased as heparin increased. Within the detection range of heparin, the concentration of heparin is linearly correlated with the fluorescence intensity of EY. According to this, a new method for the determination of trace heparin was established. The detection limit of this method was 0.071 mg/L. The detection range of heparin was 0. 1 - 2.0 mg/L. For six parallel detenninations, the relative standard deviations were 1.4% -3.6% and the recoveries were 95.2% - 105.3%. The method has been applied to the determination of heparin sodium in injections, and satisfactory results have been obtained.

关 键 词：曙红 中性红 荧光能量转移 抑制 肝素 

分 类 号：O657.3[理学—分析化学]