多璧碳管／聚中性红修饰电极的制备及对鸟嘌呤和腺嘌呤的应用研究  

Fabrication of a multi-wall carbon nanotube/poly(neutral red) modified electrode and determination of adenine and guanine

作　　者：黄强[1] 刘红英[1] 张翠红[1] 方宾[1] 

机构地区：[1]安徽师范大学化学与材料科学学院,安徽省化学生物传感重点实验室,芜湖241000

出　　处：《分析试验室》2009年第9期31-34,共4页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(20675001);安徽省教育厅自然科学基金(2006kj1198B)项目资助

摘　　要：结合纳米材料的电催化特性和中性红聚合物薄膜的分子识别能力，以玻碳电极为基体制备了多壁碳管／聚中性红（MWNT／PNR）修饰电极，并用表面扫描电镜和循环伏安法进行了表征。实验表明，该修饰电极对腺嘌呤（A）和鸟嘌呤（G）都表现出了良好的电催化性能。在最佳条件下，用示差脉冲伏安法对A和C进行了测定，其氧化峰电流于A和G的浓度分别在0．01～4gmol／L和0．01～8pmol／L范围内呈良好的线性关系，检测限均为5×10^-9mol／L（S／N=3）。该修饰电极可以用来同时测定DNA中的A和G。A multi-wall carbon nanotube/poly （neutral red） glassy carbon electrode （MWNT/PNR/GCE）, which combined the electrocatalytic property of nanomaterials with the molecular recognition property of neutral red, was fabricated by electropolymerizing neutral red onto the surface of MWNTs modified glassy carbon electrode. The modified elec- trode was characterized with scanning electron microscopy （SEM） and cyclic vohammetry （CV）. The currents were proportional to the concentrations of adenine （A） and guanine （G） in the ranges of 0.01 - 4umol/L and 0.01 - 8 umol/L. The detection limits for individual measurement of G and A were both 5 × 10^-9 mol/L. The pyrimidine bases did not interfere with the determination of A and G. This electrode could be used for the simultaneous determination of A and G in DNA.

关 键 词：多璧碳管 中性红 鸟嘌呤 腺嘌呤 修饰电极 

分 类 号：O657.1[理学—分析化学]