文心兰品种变异RAPD分子检测技术的建立及应用  

Establishment and Application of RAPD Marker Detection Technology in Oncidium Variation

作　　者：罗远华[1] 莫饶[1] 蔡林宏[1] 邓小果[1] 李江渝[1] 黄明忠[1] 

机构地区：[1]海南大学农学院,海南儋州571737

出　　处：《热带农业科学》2009年第7期28-31,42,共5页Chinese Journal of Tropical Agriculture

基　　金：国家科技支撑计划项目(No.2007BAD45B07)

摘　　要：通过对DNA提取方法的改良,随机引物的选择、RAPD反应条件的筛选,建立文心兰品种变异RAPD分子标记检测技术。利用该技术对3个母本园品种和1个试管苗品种进行RAPD检测,结果是品种"黄金2号"和"黄金3号"未发现变异;7个引物对品种"新奇士"共扩增出63条清晰带,3个样品带型发生变化,样品变异率为10%;14个引物对"黄金2号"试管苗共扩增出119条清晰带,9个样品带型发生变化,样品变异率为9.4%。结果表明,该技术能有效用于文心兰品种的变异检测。A RAPD molecular technology for detection of genetic variation of Oncidium was established through the improvement of DNA extraction methods and selection of random primers and RAPD reaction conditions. With this technology, 30ncidium varieties and an in vitro Oncidium variety were detected using the RAPD marker. Oncidium varieties Golden No.2 and Golden No. 3 were not found to have genetic variation. From the variety Sunkist a total of 63 clear bands were amplified by 7 primers, and 3 samples varied in the bands, with a genetic variation rate of the samples being 10%. From the Golden No.2 in vitro, a total of 119 clear bands were amplified by 14 primers, 9 samples changed in the bands, and the genetic variation rate of the samples 9.4%. The results showed that this technology can effectively detect the genetic variation of Oncidium.

关 键 词：文心兰 品种 试管苗 RAPD 遗传变异 

分 类 号：S682.31[农业科学—观赏园艺]