丽江双团棘胸蛙热驯化中HSP70表达量的变化  

Changes in Expression Level of HSP 70 Protein of Paa yunnanensis in Lijiang under Thermal Acclimation

作　　者：张晶晶[1] 朱静[1] 林钟婷[1] 

机构地区：[1]西南林学院保护生物学学院,云南昆明650224

出　　处：《西南林学院学报》2009年第4期42-46,共5页Journal of Southwest Forestry College

基　　金：国家自然科学基金重大研究计划项目(90411002)资助;云南省野生动植物保护与利用重点学科资助

摘　　要：用Western Blotting方法检测在不同温度梯度及不同时间热驯化下,丽江双团棘胸蛙肝脏、心脏、脑及皮肤等器官组织HSP70的表达情况。结果表明:丽江双团棘胸蛙各器官HSP70表达量与热驯化温度和时间长短有关,在15,20,30,35℃热驯化过程中,各温度条件下各器官的HSP70表达水平有差异,表明热效应会影响活体中HSP70蛋白的表达水平。The expression of HSP 70 protein in the liver, heart, encephalon and skin of Paa yunnanensis under the different heat acclimation temperature and varied time duration was tested by Western Blotting. The results showed that the expression of HSP 70 in different organs of P. yunnanensis in Lijiang was associated with the tem- perature and the duration of thermal acclimation. There were differences in the expression level of HSP 70 under 15 ℃ ,20℃ ,30℃and 35 ℃ in different organs, indicating that thermal acclimation could affect the expression level of HSP 70 in the living organism of P. yunnanensis.

关 键 词：双团棘胸蛙 热驯化 HSP70 

分 类 号：S718.62[农业科学—林学] Q95[生物学—动物学]