白藜芦醇甙减轻缺血/再灌注诱发的大鼠心肌细胞凋亡(英文)  

Polydatin attenuates ischemia/reperfusion-induced apoptosis in myocardium of the rat

作　　者：张利萍[1] 马会杰[1] 布会敏[1] 王妹玲[1] 李茜[1] 齐钊[1] 张翼[1] 

机构地区：[1]河北医科大学生理学教研室,石家庄050017

出　　处：《生理学报》2009年第4期367-372,共6页Acta Physiologica Sinica

摘　　要：本研究旨在探讨白藜芦醇甙(polydatin)对大鼠缺血/再灌注心肌细胞凋亡的影响及其相关机制。成年雄性Sprague-Dawley大鼠随机分为三组:对照(CON)组,缺血/再灌注(ischemia/reperfusion,I/R)组,白藜芦醇甙(POLY,50μmol/L)组。应用Langendorff灌注技术,给予心脏30min缺血/60min再灌注,通过TUNEL和流式细胞技术检测细胞凋亡以及Bcl-2和Bax蛋白的表达。结果显示:(1)与CON组相比,I/R组心肌缺血/再灌注后心肌凋亡细胞(TUNEL染色)数量、凋亡率明显增加;(2)与I/R组相比,POLY组心肌缺血/再灌注后心肌凋亡细胞(TUNEL染色)数量明显减少[(18.1±4.0)%vs(35.1±5.4)%,P<0.01];(3)流式细胞术测定显示I/R组心肌细胞凋亡率为(15.43±4.55)%,明显高于POLY组心肌[(8.66±3.18)%](P<0.01);(4)I/R组心肌Bax蛋白表达明显高于POLY组心肌(P<0.05),POLY组心肌Bcl-2蛋白表达及Bcl-2/Bax比值明显高于I/R组心肌(P<0.05,P<0.01)。以上结果表明,白藜芦醇甙可通过增加Bcl-2蛋白表达,减少Bax蛋白表达而减少I/R诱导的大鼠心肌细胞凋亡。The aim of the present study was to investigate the effect of polydatin on apoptosis induced by ischemia/reperfusion （I/R） in rat myocardium and to explore the underlying mechanism. Adult male Sprague-Dawley （SD） rats were randomly divided into control, I/R and polydatin （50 μmol/L） groups. On the Langendorff apparatus, isolated rat heart was subjected to 30-min global ischemia followed by 60-min reperfusion. TUNEL labeling and flow cytometric techniques were used for the measurement of apoptosis and the expression of Bcl-2 and Bax protein in cardiomyocytes of rat. The results showed： （1） Compared with those in the control group, the number of TUNEL-positive cells and apoptosis rate were increased in I/R group; （2） Compared with that in the I/R group, the number of TUNEL-positive cells was significantly decreased in the polydatin group [（18.1±4.0）% vs （35.1±5.4）%, P〈0.01 ]; （3） Apoptosis rate assayed by flow cytometry in I/R group was significantly higher than that in polydatin group [（15.43±4.55）% vs （8.66±3.18）%, P〈0.01]; （4） Expression level of Bax protein was higher in UR group than that in polydatin group （P〈0.05）, while the level of Bcl-2 protein and Bcl-2/Bax ratio were higher in polydatin group than those in I/R group （P〈0.05, P〈0.01）, respectively. The results obtained suggest that polydatin exerts an inhibitory effect on I/R-induced apoptosis through increasing Bcl-2 protein expression and decreasing Bax protein expression in myocardium of the rat.

关 键 词：白藜芦醇甙 细胞凋亡 缺血/再灌注 心脏 大鼠 

分 类 号：R285.5[医药卫生—中药学]