乙醇导致小鼠生精细胞慢性损伤与FasL表达的关系  

Relation of the Chronic Damage of Testicular Germ Cells and Its Fas Ligand Expression Induced by Ethanol

作　　者：章劲夫[1] 桂亚平[1] 胡佳华[2] 

机构地区：[1]同济大学附属同济医院泌尿外科,上海200065 [2]中国科学院上海生命科学研究院生物化学与细胞生物学研究所,上海200031

出　　处：《细胞生物学杂志》2009年第4期559-562,共4页Chinese Journal of Cell Biology

摘　　要：为研究乙醇摄入导致睾丸组织生精细胞慢性损伤的机制,采用野生型(WT)小鼠和广泛表达人Fas配体(Fas ligand,FasL)的转基因型(TG)小鼠分别用20%(V/V)乙醇喂养12周,应用苏木精-伊红(HE)染色、RT-PCR和Western印迹检测等方法观察了乙醇处理后小鼠生精细胞损伤和FasL表达的变化。HE染色结果显示乙醇喂养后,野生型鼠睾丸组织形态基本正常,而转基因鼠曲细精管有明显退行性上皮空泡化现象;RT-PCR和Western印迹检测结果显示乙醇喂养12周的WT小鼠和TG小鼠睾丸FasL表达高于对照组(普通饮用水喂养12周),两组各自相比有统计学意义(P<0.05)。研究提示FasL可能参与乙醇摄入引起的睾丸组织生精细胞慢性损伤过程,其作用机制可能是启动Fas/FasL调控生精细胞凋亡。To explore the mechanisms of chronic damage of testicular germ cells induced by chronic ethanol on the transgenic mice ubiquitously expressing human FasL, both wild type （WT） mice and transgenic （TG） mice were fed with ethanol （20% V/V） for 12 weeks. The histological changes of testicular germ ceils were assessed by HE dying, and the Fas ligand （FasL） expression was detected using RT-PCR and Western blot. The result showed that there was a degenerative change in epithelial component of the tubules in TG mice whereas a normal shape in that of WT mice after a chronic ethanol feeding for both, and the FasL was up-regulated in the testis of ethanol-treated TG and WT mice compared with corresponding vehicle-treated control mice （P〈0.05） after a 12- week treatment. These evidences suggest that the up-regulation of FasL may be involved in the chronic damage of testicular germ cells induced by chronic ethanol, and Fas/FasL could act as a regulator for the apoptosis of testicular germ cells.

关 键 词：FAS配体 乙醇 细胞凋亡 睾丸 转基因小鼠 

分 类 号：R114[医药卫生—卫生毒理学]