Phylogenetic investigation on five genera of tintinnid ciliates (Ciliophora, Choreotrichia), based on the small subunit ribosomal RNA gene sequences  

作　　者：Zicong Li Zhenzhen Yi Jinpeng Yang Jun Gong John C. Clamp Khaled A.S. Al-Rasheid Saud Al-Arifi Abdulaziz A. Al-Khedhairy Weibo Song 

机构地区：[1]Laboratory of Protozoology, KLM, Ocean University of China, Qingdao 266003, China [2]Department of Zoology, Natural History Museum, London SW7 5BD, UK [3]Department of Biology, North Carolina Central University, NC 27707, USA [4]Zoology Department, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia

出　　处：《Progress in Natural Science:Materials International》2009年第9期1097-1101,共5页自然科学进展·国际材料（英文版）

基　　金：supported by the National Natural Science Foundation of China (30870280 and 30870264);a joint grant from the Center of Excellence in Biodiversity and the DNA Research Chair, King Saud University

摘　　要：The phylogenetic relationship among tintinnid ciliates are relatively poorly studied based on molecular data. In the present work, seven species belonging to five genera of the order Tintinnida (Amphorellopsis acuta, Codonellopsis nipponica, Favella taraikaensis, Steno-semella nivalis, Tintinnopsis beroidea, Tintinnopsis cylindrica and Tintinnopsis lohmanni) were analyzed using the information on their small subunit ribosomal RNA gene sequences. Phylogenetic trees were constructed using Bayesian inference (BI), maximum parsimony (MP), neighbor-joining (NJ), and least-squares (LS) methods. Generally, similar topologies were revealed with high or moderate supports, in which the main results show that (1) all tintinnids analyzed belong to a single assemblage; (2) congeners in Tintinnopsis do not cluster together, which indicates that the lorica-based definition for this genus is not consistent with the SSU rRNA phylogeny; (3) A. acuta groups with Tintinnidium mucicola but not with Eutintinnus, indicating that the traditional family Tintinnidae might be a paraphyletic group; (4) Stenosemella and Codonellopsis are clearly most related and possibly even merged into one genus regarding their similar morphology and molecular analyses, and possession of a hyaline collar is the only characteristic of the genus.The phylogenetic relationship among tintinnid ciliates are relatively poorly studied based on molecular data. In the present work, seven species belonging to five genera of the order Tintinnida （Amphorellopsis acuta, Codonellopsis nipponica, Favella taraikaensis, Stenosemella nivalis, Tintinnopsis beroidea, Tintinnopsis cylindrica and Tintinnopsis lohmanni） were analyzed using the information on their small subunit ribosomal RNA gene sequences. Phylogenetic trees were constructed using Bayesian inference （BI）, maximum parsimony （MP）, neighbor-joining （NJ）, and least-squares （LS） methods. Generally, similar topologies were revealed with high or moderate supports, in which the main results show that （1） all tintinnids analyzed belong to a single assemblage; （2） congeners in Tintinnopsis do not cluster together, which indicates that the lorica-based definition for this genus is not consistent with the SSU rRNA phylogeny; （3） A. acuta groups with Tintinnidium mucicola but not with Eutintinnus, indicating that the traditional family Tintinnidae might be a paraphyletic group; （4） Stenosemella and Codonellopsis are clearly most related and possibly even merged into one genus regarding their similar morphology and molecular analyses, and possession of a hyaline collar is the only characteristic of the genus.

关 键 词：PHYLOGENY Tintinnida Molecular systematics SSU rRNA 

分 类 号：Q965.8[生物学—昆虫学] Q959.468