高糖对系膜细胞产生纤维联接蛋白、Ⅳ型胶原及PAF的影响  

Effect of high glucose on the role of fibronectin, collagen IV and platelet activating factor of mesangial cells

作　　者：周素娴[1] 雷闽湘[1] 赵晋晋[1] 

机构地区：[1]中南大学湘雅医院内分泌科,湖南长沙410008

出　　处：《中国医师杂志》2009年第8期1024-1026,共3页Journal of Chinese Physician

摘　　要：目的探讨高浓度葡萄糖条件下，系膜细胞产生纤维联接蛋白、IV型胶原、血小板活化因子（PAF）的变化。方法人系膜细胞单独培养，分为对照组、甘露醇组、高糖组、BN52021组，ELISA法检测各组细胞上清液中纤维联接蛋白（Fn）、Ⅳ型胶原（Col Ⅳ）、PAF含量，实时荧光定量检测系膜细胞血小板活化因子受体（PAF-RmRNA）表达。结果高糖刺激下，系膜细胞培养上清液Fn和colⅣ含量升高（P均〈0．05），BN52021可抑制高糖刺激的Fn和colⅣ含量升高（P〈0．05）。高糖可引起系膜细胞培养上清液PAF含量升高（P均〈0．05）。高糖可上调系膜细胞PAF-R mRNA表达（P均〈0．05）。结论（1）高糖刺激系膜细胞Fn、ColⅣ、PAF产生，高糖刺激的Fn、Col Ⅳ分泌增加部分与PAF有关。（2）高糖可促进系膜细胞PAF-R基因表达，放大PAF的生物效应。Objective To study the effect of high glucose on the role of fibronectin,collagen IV and platelet activating factor（PAF） of mesangial cells. Methods The mesangial cells were divided into 4 groups： Control, mannitol, high glucose and BN52021 s group. Mesangial ceils were cultured in DMEN with or without BN52021 in high glucose media up to 24 hours. The levels of fibronectin, collagen IV and PAF were determined by enzyme linked immunosorbent assay in the culture media. The expression level of platelet activating factor receptor（PAF-R） in mesangial cells was determined by real-time fluorescence quantitative PCR. Results The levels of fibronectin and collagen IV of high glucose group were higher than those of control group （ P 〈 O. 05 ）. The levels of fibronectin and collagen IV of the BN52021 group were lower than those of high glucose （ P 〈 O. 05 ）. The level of PAF of high glucose was markedly higher than that of control group （ P 〈 O. 05 ）. The expression level of PAF-R in mesangial cells of high glucose was higher than that of control group （ P 〈 0. 05 ）. Conclusions High glucose can promote mesangial cells to produce fibronectin, collagen IV and PAF. The increase of fibronectin and collagen IV is partly related with PAF. The expression of PAF-R in mesangial cells is promoted by high glucose, which can enlarge the biological effect of PAF.

关 键 词：葡萄弗萝/药理学 肾小球膜/药物作用/细胞学 纤连蛋白类/药物作用 胶原Ⅳ型/药物作用 血小 板活化因子/药物作用 

分 类 号：R692[医药卫生—泌尿科学] R587[医药卫生—外科学]