嗅成鞘细胞移植与甲基强的松龙联合应用对急性脊髓损伤大鼠功能恢复和Nogo-A表达的影响  

Effects of combining olfactory ensheathing cells and methylprednisolone treatment on the functional recovery and Nogo-A expression following acute spinal cord injury in rat

作　　者：王锋[1] 王玮[1,2] 

机构地区：[1]福建医科大学基础医学院人体解剖学与组织学胚胎学系,福州350004 [2]福建医科大学基础医学院神经生物学研究中心,福州350004

出　　处：《解剖学杂志》2009年第4期443-447,共5页Chinese Journal of Anatomy

基　　金：福建省科技计划项目（项目编号2005K042）

摘　　要：目的：探讨嗅成鞘细胞（OECs）移植联合应用甲基强的松龙（MP）对急性脊髓损伤大鼠神经功能恢复及Nogo-A表达的影响。方法：自新生SD大鼠嗅球取材，纯化培养嗅成鞘细胞；建立大鼠脊髓挫伤模型，术后1、3、7、14d，参照BBB法进行各组神经功能检测，取脊髓组织，免疫组织化学及RT-PCR法检测Nogo—A表达。结果：OECs组和MP组在7、14d BBB评分高于SCI组，但低于OECs＋MP组；免疫组织化学和RT-PCR显示：在3、7、14d，OECs组和MP组Nogo-A的表达量与SCI组相比，均呈明显下调；但其下调Nogo-A效应低于OECs＋MP组。结论：OECs移植联合应用MP可明显改善大鼠急性脊髓损伤后神经功能恢复，其机制部分与下调Nogo-A表达有关；OECs移植与MP联合应用在促进SCI后神经功能恢复方面要优于单独使用OECs或MP，两者之间可能存在协同效应。Objective： To investigate the effect of combining olfactory ensheathing cells （OECs） and large dose of methylprednisolone （MP） on Nogo-A expression and recovery of its neurological functions after acute spinal cord injury （SCI） in rat. Methods： OECs were harvested and purified following a previously described Nash＇s technique. Rat acute spinal cord injury model was established and were randomly divided into 6 groups： a normal group, a sham-operated group, SCI group, OECs-treated group, MP-treated group, and OECs＋MP-treated group. Before being sacrificed and at 1 d, 3 d, 7 d and 14 d after SCI, neurological functions were evaluated by using the Basso-Beattie-Bresnahan （BBB） ; The Nogo-A was detected by the immunohistoehemical method. Reverse transcription-polymerase chain reaction （RT-PCR） were used to measure the expression of Nogo-A at 1 d, 3 d, 7 d and 14 d after SCI. Results： Compared with the control group, OECs-treated and the MP-treated groups, the BBB score of the MP＋OECs-treated group was higher at 7 d and 14 d after SCI and the expression of Nogo-A around the injury area was significantly down-regulated at 3 d, 7 d and 14 d after SCI. Conclusion： Transplantation of OECs combining with MP after acute spinal cord injury in rat can significantly improve the neurological functions, of which its mechanism was partly related to down-regulation in the expression of Nogo-A. Combination of OECs and MP has better effect on the recovery of neurological functions, which suggest that OECs and MP may have synergistic effects.

关 键 词：嗅成鞘细胞 甲基强的松龙 脊髓损伤 Nogo—A 大鼠 

分 类 号：R651[医药卫生—外科学] R774.6[医药卫生—临床医学]