结缔组织生长因子对肾性高血压大鼠心肌纤维化的影响及机制研究  

Effect and mechanism of connective tissue growth factor on myocardium fibrosis of renal hypertensive rat

作　　者：陈智龙[1] 田青[1] 刘莉[1] 汪燕舞[2] 毕朝武[3] 毕朝芳[1] 

机构地区：[1]湖北省黄石市中心医院老年病区,湖北黄石435000 [2]湖北省黄石市中心医院病理科,湖北黄石435000 [3]湖北省黄石市中心医院湖北省黄石市爱康医院心内科,湖北黄石435000

出　　处：《中国医师杂志》2009年第8期1052-1055,共4页Journal of Chinese Physician

基　　金：湖北省黄石市医学卫生科技立项资助项日（2006-1）

摘　　要：目的从分子水平探讨结缔组织生长因子（CTGF）在肾血管性高血压大鼠左心室重构发生、发展中的作用。方法构建肾血管性高血压大鼠（RHR）模型，并以假手术实验大鼠（SOR）为对照，运用免疫组化法对CTGF、TGF-β1在左室心肌的分布及表达进行半定量分析；用RT-PCR法检测心肌组织CTGF mRNA、TGF-β1mRNA；并观察左室心肌胶原形态，检测胶原容积分数、血清I型前胶原羧基端前肽和胶原浓度。结果在同周龄RHR组CVF、PJP、Coll明显高于SOR组（P〈0．01）；在同周龄RHR组左室心肌中的CTGF、TGFβ1表达和CTGF mRNA、TGFβ1mRNA较SOR组明显增强（P〈0．05）；在RHR和SOR组左室心肌中的cTGF、TGF-β1表达12周大鼠明显高于6周大鼠（P〈0．05）；相关分析表明，CTGF与CTGF mRNA（r=0．815，P〈0．01）、TGF-β1（，=0．626，P〈O．05）、CVF（r=0．742，P〈0．01）、Coll（r=0．793，P〈0．01）、PICP（r=0．675，P〈0．01）均呈正相关。结论CTGF参与了肾血管性高血压大鼠心肌纤维化的形成。Objective To investigate the roles of CTGF in the development of myocardial fibrosis of renovascular hypertensive rats from molecular level. Methods Renovascular hypertensive rat （RHR） animal models were constructed. Sham operational rats were used as control group. CTGF, TGF-β1 were evaluated by qualitative and semiquantitative immunohistochemical staining in the sixth and the twelfth week after operation to explore their distribution and expression in left ventricular tissue., CTGF mRNA, TGF-β1 mRNA were evaluated by RT-PCR, and the total collagen in left ventricular interstitial tissue were observed, CVF, PIP and Coil were also measured. Results Compared with sham operated rat, CVF, PIP and Coll carboxypropetide of precollagen I and collagen concentration in renovaseular hypertensive rat were higher（ P 〈0. 01 ）. The expression of CTGF, TGF-β1 and CTGF mRNA, TGFβ1 mRNA in myocardium of left ventricle of RHR were higher than those in SOR, CTCF primarily reacted with myocardial interstitium and artery. TGF-β1 mainly expressed in myocardial cells. The expression of CTGF,TGF-β1 and CTGF mRNA, TGFβ1mRNA in myocardium of left ventricle of the twelfth week RHR or SHR were higher than that in the sixth RHR or SHR（ P 〈 0＋ 05 ）. Correlative analysis revealed that CTGF was positively correlated with （ r = O. 815, P 〈0. 01） ,TGF-β1（ r =0. 626, P 〈0. 05） ,CVF（ r =0. 742, P 〈0. 01） ,Coll （ r =0. 793, P 〈0.01） and PICP（ r =0.673, P 〈 0. 01 ）. Conclusions CTGF may play an important role in the development of myocardial interstitial fibrosis.

关 键 词：胞间信号肽类和蛋白质类/药理学 高血压 肾性/药物疗法 心内膜心肌纤维化症/药物疗法 

分 类 号：R285.5[医药卫生—中药学]