缬沙坦对大鼠心肌缺血再灌注损伤P-选择素的影响  

Effects of valsartan on p-selectin in myocardial ischemia reperfusion injury of rat

作　　者：马国强 李跃荣[2] 张颖懿 宫延荣 

机构地区：[1]山东省乳山市人民医院心血管内科,山东乳山264500 [2]滨州医学院附属医院心血管内科

出　　处：《中国医师杂志》2009年第8期1063-1065,共3页Journal of Chinese Physician

摘　　要：目的通过结扎冠状动脉制作大鼠心肌缺血再灌注模型，观察血管紧张素Ⅱ-1型受体（AT1）拮抗剂缬沙坦（valsar-tan）对大鼠心肌缺血再灌注损伤（MIRI）P-选择素影响。并初步探讨其作用机制。方法90只Wister大鼠随机分为4组：l组：假手术组（10只），2组：缺血组（20只），3组：缺血再灌注组（30只），4组：缬沙坦组（30只）。其中1，2，3组用生理盐水2ml每天灌胃，4组用缬沙坦20mg／kg每天灌胃。共灌胃4周制作模型。1组只穿线不结扎。2组分别结扎左冠状动脉前降支30和60min各10只。3组结扎冠状动脉前降支30min后分别再灌注60、120和360min各10只。4组结扎冠状动脉前降支30min后分别再灌注60、120和360min各10只。酶联免疫吸附法测定不同时间血清P-选择素浓度。观察缬沙坦对大鼠心肌缺血再灌注心肌的保护作用。结果在缬沙坦组及缺血再灌注组P-选择素逐渐升高（P〈0．05或P〈0．01）。缬沙坦组较缺血再灌注组P-选择素降低（P〈0．01）。缬沙坦可以减少P-选择素的释放。结论缬沙坦可以减轻MIRI，可能通过减少P-选择素的释放，减轻心肌细胞的损伤。Objective To observe the effects of angiotensin Ⅱ type 1 receptor blocker, valsartan on the release of P-selectin in rat model of myocardial ischemia reperfusion injury, and the preliminary action mechanism of valsartan. Methods Ninety wister rats were randomly divided into four groups, Group I （ Sham operated group, n = 10 ） , Group Ⅱ （Ischemic group, n = 20 ） , Group m （Ischemic reperfusion Group, n = 30） and Group 1V （Valsartan group, n = 30）. Group IV was given valsartan by direct gastric garages 20rag/（ kg ·d）. Group I , Ⅱ, Ⅲ were given normal saline garages 2ml/d）. After four weeks of pretreatment, the middle point of left anterior coronary artery （LAD） was sham ligated for 30 minute（ Group I ）. The middle point of left anterior coronary artery （LAD） was ligated for 30 minutes （ n = 10 ） and 60 rain （ n = 10） in Group II. The middle point of left anterior coronary artery （LAD） was ligated for 30 minutes, followed by 60 minutes （ n = 10） , 120 minutes（ n = 10） , 360minutes reperfusion（ n = 10） in GroupⅢ. The middle point of left anterior coronary artery （LAD） was ligated for 30 minutes, followed by 60 minutes（ n =10）, 120 minutes（ n = 10）, 360minutes reperfusion （ n = 10） in Group IV. Blood samples were taken from right atrium for measurement. The contents of p-selectin were detected by the method of Enzyme-Linked Immunosorbnent Assay （ELISA）. Results The expression of P-seleetin continually elevated after reperfusion（ P 〈 0. 05 or P 〈0. 01 ）. P-selectin level in group Ⅲ was higher than that in group IV（ P 〈0. 01 ）. The valsartan could reduce the release of p-selectin. Conclusions Valsartan could relieve myocardial ischemia reperfusion injury of rat, which may be through reducing p-selectin of plasma.

关 键 词：缬氨酸/类似物和衍生物/药理学 心肌再灌注损伤/预防和控制/代谢 P选择素/药物作用/代谢 

分 类 号：R54[医药卫生—心血管疾病] R9[医药卫生—内科学]