胸腺基质淋巴细胞生成素在变应性鼻炎模型小鼠鼻黏膜中的表达  

Expression of thymic stromal lymphopoietin in nasal mucosa of a mouse model with allergic rhinitis

作　　者：许梅莉[1] 郭永清[2] 

机构地区：[1]厦门大学附属中山医院综合ICU科,厦门361004 [2]厦门大学附属中山医院耳鼻咽喉科

出　　处：《临床耳鼻咽喉头颈外科杂志》2009年第17期794-795,799,共3页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

摘　　要：目的：探讨胸腺基质淋巴细胞生成素（TSLP）在变应性鼻炎模型小鼠鼻黏膜中的表达。方法：将20只6～8周BALB／c小鼠随机分为变应性鼻炎组和自然对照组，每组10只。以鸡卵清蛋白致敏建立变应性鼻炎模型。组织标本石蜡包埋，采用免疫组织化学方法和荧光PCR方法检测TSLP在小鼠鼻黏膜中的表达。结果：自然对照组鼻黏膜未见明显TSLP表达，变应性鼻炎组鼻黏膜可见明显TSLP表达。结论：TSLP在变应性鼻炎的鼻黏膜中表达。Objective：To investigate the expression of thymic stromal lymphopoietin（TSLP） in the nasal mucosa of mouse with allergic rhinitis. Method：Twenty wide type BALB/c mouse were divided into 2 groups randomly. Two groups were included allergic rhinitis group（group A） and control group（group B）. The mouse model of allergic rhinitis was established by ovalbumin（OVA） sensitization and challenge. The expressions of TSLP in the nasal mucosa was determined by realtime quantitative PCR and immunohistochemical method. Result：The expression of TSLP in the nasal mucosa of group A was significantly higher than that in group B（P〈0.01）. Conclusion： TSLP plays a role in the mouse model of allergic rhinitis.

关 键 词：鼻炎 变应性 胸腺基质淋巴细胞生成素 鼻黏膜 

分 类 号：R765.21[医药卫生—耳鼻咽喉科]