茶树冷胁迫诱导H1-histone基因的克隆与序列分析  

Cloning and Sequence Analysis of Cold-induced H1-histone Gene from Camellia sinensis

作　　者：房婉萍[1] 邹中伟[1] 侯喜林[1] 张定[1] 段云裳[1] 杨亦扬[1] 黎星辉[1] 

机构地区：[1]南京农业大学园艺学院,南京210095

出　　处：《西北植物学报》2009年第8期1514-1519,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(30800884);教育部高校博士点基金(20060307024;20070307020);南京农业大学青年科技创新基金(KJ07008);常州市科技攻关项目(CE2007210);中国博士后科学基金(20070411061);江苏省科技攻关计划(BE2007301;BE2008320-2);国家"十一五"科技支撑计划(2006BAD06B01);苏州市科技攻关计划(SNG0809;WNZ0802);江苏省三项工程(sx2007g17;sx2008g10)

摘　　要：利用cDNA-AFLP技术对茶树不同低温胁迫处理下的基因表达差异进行了分析,获得一低温诱导后特异表达片段TDF8(transcript derived fragment,TDF)。BLAST比对结果显示,该片段与其它物种的逆境诱导特异表达的H1-histone基因有很高的相似性。通过RACE分别扩增出其3′和5′末端序列,成功获得该基因cDNA全长序列(GenBank登录号EU716314)。所得序列全长916 bp,其开放阅读框共编码207氨基酸,蛋白分子量约为30.77 kD。该基因氨基酸序列分析表明,与烟草的胁迫诱导H1-histone基因的氨基酸序列一致性达79%。可以认为,该基因应该属于逆境诱导条件下表达的H1-histone基因家族成员之一。To obtain insights to the comprehensive molecular characteristics relating to the cold resistant mechanisms of tea plant,we performed a large-scale screening of cold-induced genes in tea plant by cDNA- AFLP. A cDNA fragment, TDF8 encoding a stress-induced Hl-histone homolog was isolated and identified. The complete cDNA sequence was cloned by RACE （rapid amplification of cDNA ends）, named Camellia sinensis cold-induced Hl-histone （CSH1-Histone） gene. Its full cDNA sequence is 916 bp （GenBank accession number EU716314） and contains an open reading frame encoding a polypeptide of 207 amino acid residues with a predicable molecular mass of 30.77 kD. By using the program of Blast on GenBank database,the sequence presents a high match with stress-induced Hl-histone genes from other plants. We concluded that the cloned sequence is a member of Hl-histone genes from tea plant.

关 键 词：茶树 H1-histone基因 克隆 序列分析 

分 类 号：Q785[生物学—分子生物学]