利用RAPD特异标记分析东北猬草染色体组成  

Genomic Constitution of Hystrix komarovii (Poaceae:Triticeae) Inferred from Genome-specific RAPD Assay

作　　者：黄燕[1] 张海琴[1] 刘静[2] 杨瑞武[2] 周永红[1] 

机构地区：[1]四川农业大学小麦研究所,四川温江611130 [2]四川农业大学生命科学与理学院,四川雅安625014

出　　处：《西北植物学报》2009年第8期1538-1543,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(30670150;30870154);四川省教育厅和科技厅重点项目

摘　　要：选用5个染色体组特异的RAPD引物(St、H、Ns、Ee、Eb),对东北猬草[Hystrix komarovii(Roshev.)Ohwi]等5个猬草属及其8个近缘属物种进行PCR扩增,以探讨东北猬草的染色体组组成。结果显示:Hy.kom-arovii具有Ns染色体组特异的RAPD标记,而没有St、H、Ee和Eb特异的RAPD标记。表明Hy.komarovii含有Ns染色体组,而不含St和H染色体组,认为其染色体组组成可能与Hy.duthiei、Hy.coreana和Leymus arenarius一样,具有NsXm染色体组。根据染色体组分类原理,支持将东北猬草归于赖草属中。To determine the genomic constitution of Hystrix komarovii （Roshev.） Ohwi,five Hystrix species and eight relatives were analyzed by genome-specific RAPD assay. Five gemome-specific RAPD markers representing the St, H, Ns, E^e and E^b genome, respectively, were selected for the PCR amplification. The results showed that Hy. komarovii had the Ns genome-specific RAPD markers,and was absent the St, H, E^e and E^b genome-specific RAPD markers. It indicated that Hy. komarovii might contain the Ns genome, and have no St or H genome. Thus, Hy. komarovii might contain the NsXm genomes, which was similar with the those of Hy. duthiei,Hy, coreana and Leymus arenarius. Hy. komarovii should be transferred to the genus Leymus according to the genomic system of classification in Triticeae.

关 键 词：猬草属 东北猬草 赖草属 染色体组 特异RAPD标记 

分 类 号：Q789[生物学—分子生物学]