ADSL和GARS-AIRS-GART基因多态位点及聚合基因型对白耳鸡胸肌肌苷酸(IMP)含量的效应分析  

Effects of Single and Pyramiding Genotypes of ADSL and GARS-AIRS-GART Genes on Baier Chicken Muscle Inosine Monophosphate(IMP) Contents

作　　者：韩威[1] 张学余[1] 李慧芳[1] 束婧婷[1] 李国辉[1] 朱云芬[1] 

机构地区：[1]中国农业科学院家禽研究所,扬州225003

出　　处：《农业生物技术学报》2009年第4期577-581,共5页Journal of Agricultural Biotechnology

基　　金：国家科技支撑计划项目(No.2008BADB2B01);国家高技术研究与发展计划(863)项目(No.2006AA10Z1D8)资助

摘　　要：采用PCR-SSCP方法检测白耳鸡(gallus)腺苷琥珀酸裂解酶基因(ADSL)外显子2和甘氨酰胺核苷酸合成酶-5-氨基咪唑核苷酸合成酶-甘氨酰胺核苷酸转甲基酶基因(GARS-AIRS-GART)5'侧翼区多态性,分析多态位点不同基因型及聚合基因型与90日龄胸肌肌苷酸(IMP)含量的关联,并比较分析了不同聚合基因型个体的生产性能。结果表明,ADSL基因外显子2和GARS-AIRS-GART基因5'侧翼区的多态位点C3484T和C-179T与IMP含量显著相关(P<0.05),TT为两种有利单基因型,其对IMP含量的加性效应分别为0.1710和0.128mg/g,显性效应分别为-0.025和0.031mg/g。两种有利单基因型聚合个体TTTT的IMP含量显著高于其它基因型聚合个体(除TTCC型),且在聚合基因型中两种有利单基因型TT对IMP含量的影响均达到显著水平(P<0.05),但两者间的互作效应并不显著(P>0.05)。TTTT聚合基因型个体的90日龄平均体重和72周龄产蛋性能仍保持相对稳定。The polymorphism sites in exon 2 domain of adenylosuccinatelyase （ADSL） and 5＇-flanking domain of glycinamide ribonucleotide synthetase-aminoimidazole ribonucleotide synthetase-glycinamide ribonucleotide transformylase genes （GARS-AIRS-GART）on Baier chicken （gallus） were identified by PCR-SSCP. Association between the founded polymorphism sites as well as pyramiding genotypes and inosine monophosphate acid （IMP） contents in muscles were tested by least square means. The reproduction performance of individuals with different pyramiding genotypes were also analyzed. The results showed that the founded polymorphism sites C3484T and C-179T had significant correlation with IMP contents in muscles. The TTs were two advantageous genotypes. Their additive effects for ADSL and GARS-A1RS-GART genes were 0.1710 and 0.128 mg/g, and the dominant effects were -0.0250 and 0.031 mg/g, respectively. The pyramiding genotype TTTT had higher IMP contents than that of other pyramiding genotypes. The effects of two advantageous TTs in pyramiding genotype TTTT were still significant on IMP contents, however, their interaction effect was not significant （P 〉 0.05）. The average weights of 90 days and egg reproduction in pyramiding TTTT populations had no variation.

关 键 词：腺苷琥珀酸裂解酶基因(ADSL) 甘氨酰胺核苷酸合成酶-5-氨基咪唑核苷酸合成酶-甘氨酰胺核苷酸转甲基酶基因(GARS-AIRS-GART) 白耳鸡 基因聚合 肌苷酸 

分 类 号：S188[农业科学—农业基础科学]