荷斯坦牛白细胞抗原基因-DRB3(BoLA-DRB3)外显子2多态性及其与体细胞评分的关系  

Polymorphism of Exon 2 of Bovine Lymphocyte Antigen-DRB3 Gene(BoLA-DRB3) and Its Relationship with Somatic Cell Score in Holstein Cattle

作　　者：李春苗[1] 石万海[2] 储明星[3] 安永福[4] 陈宏权[1] 狄冉[2] 方丽[3] 

机构地区：[1]安徽农业大学动物科技学院,合肥230036 [2]北京奶牛中心,北京100085 [3]中国农业科学院北京畜牧兽医研究所,农业部畜禽遗传资源与利用重点开放实验室,北京100193 [4]河北省畜牧兽医研究所,保定071000

出　　处：《农业生物技术学报》2009年第4期593-598,共6页Journal of Agricultural Biotechnology

基　　金：国家“十一五”科技支撑计划课题(No.2006BAD04A10);北京市自然科学基金项目(No.6022015)资助

摘　　要：采用PCR-RFLP技术,用限制性内切酶BstYⅠ对河北省489头荷斯坦母牛和40头荷斯坦公牛白细胞抗原-DRB3基因(BoLA-DRB3)外显子2的284bp扩增产物进行多态性分析。荷斯坦母牛经BstYⅠ酶切产生3种基因型AA、AB和BB,其频率分别为0.078、0.380和0.542;荷斯坦公牛经BstYⅠ酶切产生2种基因型AB和BB,其频率分别为0.250和0.750;荷斯坦母牛和公牛在该酶切位点均处于Hardy-Weinberg平衡状态(母牛:χ2=0.45,P=0.799>0.05;公牛:χ2=0.82,P=0.665>0.05);用最小二乘法分析BoLA-DRB3基因外显子2的多态性与荷斯坦母牛体细胞评分(somatic cell score,SCS)的关系,结果表明AA基因型SCS最小二乘均值极显著低于AB基因型所对应的值(P=0.0098<0.01),极显著低于BB基因型所对应的值(P=0.00009<0.0001);AB基因型SCS最小二乘均值极显著低于BB基因型所对应的值(P=0.0096<0.01);对乳房炎抗性,AA是最有利基因型,BB是最不利基因型。研究结果表明BoLA-DRB3基因外显子2的多态性是提高奶牛乳房炎抗性的一个潜在的DNA标记。The exon 2 of bovine lymphocyte antigen-DRB3 gene（BoLA-DRB3） in 489 Holstein cows and 40 Holstein bulls from Hebei Province was amplified and a unique fragment of 284 bp was obtained. The 284 bp PCR product was digested with restriction endonuclease BstY I, and genetic polymorphism was investigated by PCR-RFLP. Three genotypes （AA, AB and BB） were found in 489 Holstein cows, and the frequency of AA, AB and BB genotypes was 0.078, 0.380 and 0.542, respectively. Two genotypes （AB and BB） were found in 40 Holstein bulls, and the frequency of AB and BB genotypes was 0.250 and 0.750, respectively. Chi-square test showed that both Holstein cows and bulls were in Hardy-Weinberg equilibrium （cows： X^2 = 0.45, P = 0.799 〉 0.05; bulls： X^2 = 0.82, P = 0.665 〉 0.05）. The relationship between the polymorphism in exon 2 ofBoLA-DRB3 gene and somatic cell score （SCS） in Holstein cows was analyzed by least square linear model. Least square mean of SCS for AA was significantly lower than that for AB （P = 0.0098 〈 0.01） or BB （P = 0.00009 〈 0.0001）, and least square mean of SCS for AB was significantly lower than that for BB （P = 0.0096 〈 0.01）. AA was the most favorable genotype, BB was the most unfavorable genotype for mastitis resistance. The results indicated that the polymorphism in exon 2 of BoLA-DRB3 gene is a potential DNA marker for improving mastitis resistance in dairy cattle.

关 键 词：荷斯坦牛 体细胞评分 BoLA-DRB3基因 PCR-RFLP 

分 类 号：S188[农业科学—农业基础科学]