牛CXC趋化因子受体1基因(CXCR1)编码区单核苷酸多态性  

Single Nucleotide Polymorphisms(SNPs) within the Coding Regions of Chemokine(C-X-C Motif) Receptor1 Gene(CXCR1) in Bos taurus

作　　者：官久强[1,2] 王洪梅[1] 王长法[1] 李秋玲[1] 帅素容[2] 黄金明[1] 侯明海[1] 仲跻峰[1] 

机构地区：[1]山东省农业科学院奶牛研究中心,济南250100 [2]四川农业大学动物科技学院,雅安625014

出　　处：《农业生物技术学报》2009年第4期603-608,共6页Journal of Agricultural Biotechnology

基　　金：国家高技术研究与发展计划(863)项目(No.2006AA10Z1D9);山东省良种工程项目(No.2006LZ10-04)资助

摘　　要：采用巢式PCR、DNA测序和CRS-PCR方法,研究了牛(Bos taurus)3个品种中国荷斯坦牛、鲁西黄牛和渤海黑牛的chemokine(C-X-Cmotif)receptor1(CXCR1)基因编码区的单核苷酸多态性(SNPs),发现了819(G/A)、995(G/A)和1008(C/T)3个SNPs,其中995(G/A)和1008(C/T)为首次报道的2个位点,995(G/A)导致氨基酸的改变Arg422His。CXCR1基因819(A/G)、995(A/G)和1008(C/T)3个位点在中国荷斯坦牛、鲁西黄牛和渤海黑牛群体的优势等位基因相同,分别为G、G和C,其等位基因频率分别为0.63/0.73/0.71、0.60/0.85/0.71和0.74/0.76/0.71。经χ2适合性检验,鲁西黄牛在995(A/G)和1008(C/T)位点达到Hardy-Weinberg平衡状态(P>0.05);渤海黑牛的所有位点均达到Hardy-Weinberg平衡状态,荷斯坦牛在3个位点均未达到Hardy-Weinberg平衡状态(P<0.05)。3个品种牛在819(A/G)和1008(C/T)位点均表现为中度多态(0.25<PIC<0.5),在995(A/G)位点,鲁西黄牛表现为低度多态,而其它2个品种牛表现为中度多态。The single nucleotide polymorphisms （SNPs） within the coding regions of chemokine （C-X-C motif） receptorl （CX- CR1） gene in three Bos taurus breeds, Holstein cattle, Luxi Yellow cattle and Bohai Black cattle, were investigated by nested-PCR, DNA sequencing technology and CRS-PCR method. Sequencing results showed that three SNPs were identified to be 819（G/A）,995 （G/A） and 1008（C/T）, respectively. 819（G/A） and 1008（C/T） were nonsense mutations, but 995 （G/A） resulted amino acids alteration Arg422His. In Holstein cattle, Luxi Yellow cattle and Bohai Black cattle, G, G and C predominan allele were identical at 819（G/A）, 995（G/A） and 1008（C/T）, and the allelic frequencies were 0.63/0.73/0.71, 0.60/0.85/0.71 and 0.74/0.76/0.71, respectively. Chi-square test indicated that 995 （G/A） and 1008 （C/T） of Luxi Yellow cattle and all the sites of Bohai Black cattle were in accordance with the Hardy-Weinberg equilibrium （P 〉 0.05）, while all polymorphic sites were not meet Hardy-Weinberg equilibrium （P 〈 0.05） in Chinese Holstein cattle. The value of polymorphism information content indicated that 819 （G/A） and 1008 （C/T）s were moderate polymorphism in the three breeds （0.25 〈PIC 〈0.5 ）, while 995 （G/A） were slight polymorphism in Luxi Yellow cattle and were moderate polyrnorphism in the other two breeds.

关 键 词：牛 CXCR1 单核苷酸多态性 优势等位基因 

分 类 号：S188[农业科学—农业基础科学]