小麦蓝矮病植原体溶血素基因的分离及蛋白结构功能预测  

Isolation and Bioinformatics Analysis of Hemolysin Gene from Wheat Blue Dwarf Phytoplasma

作　　者：侯伟[1] 武科科[1] 李毅然[1] 吴云锋[1] 魏婷[1] 纪玲玲[1] 

机构地区：[1]西北农林科技大学植物保护学院,陕西省农业分子生物学重点实验室,植保资源与病虫害治理教育部重点实验室,杨凌712100

出　　处：《农业生物技术学报》2009年第4期665-669,共5页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金项目(No.30871625);稻麦重要病毒病株系鉴定和防控技术体系研究(No.nyhyzx07-051);教育部长江学者和创新团队发展计划(No.200558);高等学校学科创新引智计划(No.B07049)资助

摘　　要：从小麦蓝矮病(WBD)植原体基因组中分离出溶血素基因,大小为1266bp,编码421个氨基酸(GenBank登录号:EU747230)。用生物信息学方法预测该蛋白在植原体侵染寄主过程中的致病作用。结果表明,该蛋白的二级结构中主要是螺旋,其次为折叠和无规则卷曲,不含转角,三级结构中含有三个功能区:DUF21(跨膜区结构域),CBS-pair-CorC-HlyC(双胱硫醚β合成酶结构域)和CorC-HlyC(转运体相关结构域)。推测WBD的溶血素蛋白是具N端前导序列的N-末端跨膜蛋白,可能通过DUF21结构域中的跨膜螺旋与寄主细胞相互作用,由CBS结构域获得寄主的ATP,经CorC-HlyC结构域转运,从而改变寄主体内ATP/ADP的浓度平衡而引发病害症状。The hemolysin gene of wheat blue dwarf （WBD） phytoplasma was isolated, which its length was 1 266 bp, encoding a predicted protein of 421 amino acids（GenBank accession No. EU747230）. And the pathogenic effect of hemolysin on the course of WBD phytoplasma invasion into the hosts was predicted by bioinformatics method. Results showed that the secondary structure main- ly contained helix, also some sheet and coil, but no turn. There were a DUF21 region, a CBS-pair-CorC-HlyC domain and a CorC-HlyC domain in this protein. It was predicted that the WBD hemolysin may import ATP from the host cell by the CBS domain and transport by the CorC-HlyC domain, then disturb the metabolic balance of the host cell and cause disease symptoms.

关 键 词：小麦蓝矮病 植原体 溶血素基因 同源性 蛋白结构功能预测 

分 类 号：S188[农业科学—农业基础科学]