响应面法优化超声波法提取补骨脂中活性成分的工艺研究  

Optimization of Ultrasonic Extraction of Active Components from Psoralea corylifolia L. Using Response Surface Methodology

作　　者：孟庆繁[1] 高璇[1] 司鹏[1] 王贞佐[1] 张桂荣[1] 滕利荣[1] 

机构地区：[1]吉林大学生命科学学院,吉林长春130012

出　　处：《林产化学与工业》2009年第4期87-91,共5页Chemistry and Industry of Forest Products

基　　金：中国医学基金会新药发展基金资助项目(20061108)

摘　　要：以补骨脂素与异补骨脂素总得率为考察指标,优化超声波法提取补骨脂中活性成分的工艺。选取提取时间、液料比和超声波功率为随机因子,在单因素试验的基础上,进行3因素3水平的Box-Behnken中心组合试验设计,以补骨脂素和异补骨脂素总得率为响应值进行响应面(RSM)分析,优化超声波法提取补骨脂中活性成分提取条件。结果显示,超声波法提取补骨脂中活性成分的最佳条件为:提取时间47.5 min,液料比5.6∶1(mL∶g),提取液20 mL,提取功率330 W,在最佳的提取条件下,补骨脂素和异补骨脂素总得率理论值为1.043%,验证值为1.047%,验证值与理论值间的相对误差为0.384%,验证值与真实值相吻合,说明响应面法优化补骨脂中活性成分超声波法提取条件可行。Psoralen and isopsoralen are the representative active components in Psoralea corylifolia L. The total yield of them was used as the criterion for optimization of ultrasonic extraction conditions of active components from P. corylifolia. Extracting time, liquid-solid ratio and ultrasonic power were selected as casual factors. Based on single-factor experiments, three-factor-three-level Box-Behnken central composite experiment design method was applied. Total yield of psoralen and isopsoralen was used as response values. Response surface analysis methodology （RSM） was used for analysis of the results of these experiments. The optimum conditions of ultrasonic extraction were as follows： extraction time 47.5 min, liquid-solid ratio 5. 6：1 （mL： g） extracting solution 20 mL, and ultrasonic power 330 W. Under these conditions, total yield of psoralen and isopsoralen was 1. 043 %, validation value was 1. 047 % , relative error was 0. 384 % ,which demonstrated the feasibility of this method.

关 键 词：响应面法 超声波提取 补骨脂 补骨脂素 异补骨脂素 

分 类 号：TQ351.0[化学工程] R931.71[医药卫生—生药学]