花生果种皮发育相关特异基因的克隆和表达分析(英文)  

Cloning and Characterization of Pericarp-and Testa-Specific, Developmentally Regulated Genes in Peanut

作　　者：张国林[1] 蔡宁波[1] 陈华[1] 曾建斌[1] 黄湘文[1] 庄伟建[1] 

机构地区：[1]福建农林大学福建省植物细胞与分子生物学重点实验室,福州350002

出　　处：《基因组学与应用生物学》2009年第4期640-644,共5页Genomics and Applied Biology

基　　金：supported by National Natural Science Fund(30571180)

摘　　要：本实验从花生果皮种仁抑制消减文库中筛选出了一个255bp的EST序列并进行了研究。构建了花生果种皮全长cDNA库并从中筛选出该基因的全长，命名为AhPSG8。此基因全长1118bp，开放阅读框第33～833个碱基，编码266个氨基酸残基组成的多肽。由生物信息学分析表明该基因编码多个活性位点蛋白，可能与细胞内DNA的转录有关；结构分析揭示出AhPSG8具有一个跨膜区，N端是一个由20个氨基酸组成的信号肽;该基因与已发表的基因序列没有明显的同源性，为一新基因；RT—PCR研究该基因在花生中的表达，结果显示该基因在果种皮中特异表达，10～40d果皮中丰富表达，推测为与花生果种皮发育相关基因。A 255 bp EST was isolated from the suppression subtractive hybridization （SSH） library of kernel and pericarp in this research, we constructed a full length pericarp and testa cDNA library, and the full length of the new gene was obtained, which was named A hPSG8, its full-length was 1 118 bp with an open reading frame from 33 bp to 833 bp. Bioinformatics analysis showed that the deduced protein contains a cross-membrane region and many active loci, which suggest its transcript action in cell. Also, the protein has a signal peptide containing 20 amino acids. This gene is a novel one which has no significant similarity with the sequences published. Expression analysis by RT-PCR revealed that this gene was specially expressed in pod and testa and largest in amount in pericarp from 10 to 40 days.

关 键 词：花生果种皮 特异基因 克隆 表达分析 

分 类 号：S565.2[农业科学—作物学] Q943.2[生物学—植物学]