5-HT及其受体在大鼠肝再生过程中的作用  

Effects of serotonin and its receptors in the course of liver regeneration in rats

作　　者：张晶[1] 杨仙珊[1] 李晓晴[1] 赵鹏[1] 丁美[1] 张立洁[1] 赵军[1] 段钟平[1] 

机构地区：[1]首都医科大学附属北京佑安医院,北京100069

出　　处：《山东医药》2009年第32期5-7,共3页Shandong Medical Journal

基　　金：北京市科委重大项目(D08050700650803);北京市丰台区科技项目(xm080150)

摘　　要：目的分析5-羟色胺(5-HT)及其受体在大鼠肝脏再生过程中的作用。方法将50只雄性Wistar大鼠随机分成实验组和对照组。实验组大鼠于肝大部分切除术后24、36、48和72 h处死,对照组行假手术。采用流式细胞技术检测大鼠肝脏增殖细胞核抗原(PCNA)表达、免疫组化法检测Ki67及5-HT表达、实时荧光定量PCR检测5-HT2A、2B受体亚型的表达。结果肝大部切除术后大鼠肝脏重量逐渐增加,PCNA和Ki67表达于术后24和36h达高峰;在肝切除术后各个时间点5-HT2A、2B受体在肝脏中的表达均显著升高,以术后36 h最高;术后36 h空肠嗜铬细胞5-HT含量高于24 h,且都高于正常大鼠。结论肝脏再生过程中5-HT合成及其受体表达均显著上调,可能与肝脏的再生有关。Objective To investigate the effects of serotonin and its receptors in the course of liver regeneration. Methods Fifty male Wistar rats were randomly divided into two groups in which forty rats of experimental group were subjected to 60% -70% partial hepatectomy and ten rats of control group were subjected to sham operation. Rats in experimental group were sacrificed at 24,48,36,72 h after partial hepatectomy. Proliferating cell nuclear antigens （PCNA） were measured by using flow cytometry and Ki67 was detected by using iinmunohistochemisty. The expression of serotonin receptor 2 subgroup 2A and 2B in liver were measured by using real-time fluorescence quantitative PCR. Serotonin in liver and jejunum was detected by means of immunohistoehemisty. Results The expression of PCNA and Ki67 peaked at 24 and 36 h after partial hepatectomy. The expression of liver serotonin receptor 2 subgroup 2A and 2B and jejunum serotonin increased and peaked at 36 h after partial hepatectomy. Conclusion During rat liver regeneration, the expression of serotonin and its receptors were upregulated, which might be related with liver regeneration.

关 键 词：大鼠 肝切除术 肝再生 受体 血清素 

分 类 号：R657.3[医药卫生—外科学]