RP-HPLC法测定金银花及金芪降糖片中的绿原酸  

Determination of chlorogenic acid content inFlos lonicerae and Jinqi Jiangtang Tablet by RP-HPLC

作　　者：杨茉[1] 周晶[1] 宁娜[1] 张金红[1] 王欣[2] 

机构地区：[1]天津医科大学药学院,天津300070 [2]天津中新药业集团股份有限公司隆顺榕制药厂,天津300457

出　　处：《现代药物与临床》2009年第4期237-240,共4页Drugs & Clinic

基　　金：天津市科技攻关项目(06YFGPSH02900)

摘　　要：目的建立同时测定金银花及金芪降糖片中绿原酸的反相高效液相色谱法。方法色谱柱为Diamondsil C18柱(250mm×4.6mm,5μm),以乙腈-0.4%磷酸溶液(13∶87)为流动相,检测波长为327nm,体积流量为1.0mL/min。结果在上述条件下测定金银花中绿原酸为2.49%,金芪降糖片中绿原酸为每片8.776mg。结论本方法操作简单、结果准确、回收率高、重现性好,可用于金银花及金芪降糖片的质量控制。Objective To establish a reversed-phase HPLC （RP-HPLC） method for the determination of chlorogenic acid content in Flos lonicerae and Jinqi Jiangtang Tablet. Methods The separation of chlo- rogenic acid was carried out by using the Diamondsil C18 column （250 minX4.6 mm, 5 μm） with the mobile phase containing aeetonitrile-0.4% orthophosphoric acid （13 ： 87） and a detective wavelength at 327 nm. The flow rate was 1.0 mL/min. Results The contents of chlorogenic acid in Flos lonicerae and Jinqi Jiangtang Tablet were 2.49% and 8. 776 rag/t, respectively. Conclusion The method is simple, accurate with good recovery and repeatability. It can be used to control the quality of Flos lonicerae and Jinqi Jiang- tang Tablet.

关 键 词：金银花 金芪降糖片 绿原酸 反相高效液相色谱法 

分 类 号：R286.0[医药卫生—中药学]