3种不同启动子构建ACC脱氨酶基因植物表达载体  

Construction of Plant Expression Vectors of ACC Deaminase Gene Using Three Promoters

作　　者：黄海泉[1,2] 黄美娟[1] 范志祥[2] 李建永[2] 刘璇[2] 刘飞虎[2] 

机构地区：[1]西南林学院园林学院,云南昆明650224 [2]云南大学生命科学学院,云南昆明650091

出　　处：《江西农业大学学报》2009年第4期706-710,共5页Acta Agriculturae Universitatis Jiangxiensis

基　　金：国家自然科学基金项目(30560095);云南省自然科学基金项目(2005C0004M);西南林学院基金项目(200522M)

摘　　要：从阴沟肠杆菌(Enterobacter cloacae)UW4菌株克隆出ACC脱氨酶(1-aminocyclopropane-1-carboxylicacid deaminase)基因连接至pGEM-Tvector上,并采用3种不同启动子(组成型表达启动子CaMV35S、花特异表达启动子CHSA及衰老特异表达启动子SAG12)分别构建ACC脱氨酶基因的植物表达载体,经PCR及酶切鉴定均已构建成功,为后期选择不同的花卉等植物材料遗传转化研究打下基础。ACC deaminase gene was cloned from Enterobacter cloacae UW4 strain and ligated into pGEM -T vector. Three different promoters （constitutive expression promoter CaMV35S, flower specific expression promoter CHSA and senescence specific expression promoter SAG12） were respectively utilized to successfully construct three plant expression vectors of ACC deaminase gene after PCR and restriction endonucleases detection, which laid the foundation for the future part of selecting proper flower plants and carrying out its genetic transformation.

关 键 词：ACC脱氨酶基因 花特异表达启动子 衰老特异表达启动子 植物表达载体构建 

分 类 号：S482.88[农业科学—农药学]