PAGE凝胶制备方法和银染方法的改进  

Improvement of the Methods for Polyacrylamide Gel Preparing and Silver Staining

作　　者：胡建斌[1] 李静[1] 袁鸣[1] 胡深[1] 李建吾[1] 

机构地区：[1]河南农业大学园艺学院,河南郑州450002

出　　处：《江西农业大学学报》2009年第4期742-745,共4页Acta Agriculturae Universitatis Jiangxiensis

基　　金：国家"863"计划子项目(2007AA10Z100);河南省农业科技成果转化项目(072201110017);河南农业大学博士启动基金项目(30400247)

摘　　要：PAGE凝胶分析技术已广泛运用于小分子量PCR产物的分离,但因操作环节多、耗时长,不利于大规模分子标记分析工作的开展。对PAGE凝胶的制备和银染方法作些改进,省去常规凝胶制备中亲和硅烷/剥离硅烷处理过程,以及常规银染过程中的固定和定影两步骤。与常规方法相比,该方法操作简便、耗时短、带谱的分辨率高,可代替常规方法用于SSR等标记的PCR产物的分离。The technique of PAGE gel analysis has been widely used in separation of low molecular weight PCR product. Due to the overmany technical steps and large time consumption, this technique is disadvantageous to carrying out molecular marker analysis in large scale. This research improved the methods for preparing PAGE gel and silver staining in that the bind - silane/repel - silane treatment in preparing PAGE gel and the two steps （fixation and stop bath） in conventional silver staining were omitted. In comparison to the conventional method, this method is technically simple, less time consuming and of higher resolution. This improved method could replace the conventional method and be used for separating the PCR product generated by SSR marker and other markers.

关 键 词：聚丙烯酰胺凝胶 制备方法 银染方法 SSR标记 

分 类 号：Q718[生物学—分子生物学]