固定化甲烷八叠球菌(Methanosarcina)研究——厌氧颗粒污泥的形成  

Study on immobilized Methanosarcina—formation of anaerobic granular sludge.

作　　者：杨秀山[1] 田沈[1] 郑颖 张伟 刘明[1] 汪洪杰 

机构地区：[1]首都师范大学生物系

出　　处：《中国环境科学》1998年第4期356-359,共4页China Environmental Science

基　　金：北京市自然科学基金

摘　　要：用实验室规模的ＵＡＳＢ反应器处理豆制品废水培养厌氧颗粒污泥。通过对颗粒污泥形成过程中的产气率、进出水ＣＯＤ、水滞留期（ＨＲＴ）、进出水挥发酸（乙酸、丙酸和丁酸）浓度、所产气体的甲烷含量等的变化分析和对颗粒污泥中优势产甲烷菌的扫描（ＳＥＭ）和透射（ＴＥＭ）电镜观察，阐明颗粒污泥的形成过程及特性。实验结果表明，消化器运转５０ｄ左右形成了颗粒污泥，厌氧颗粒污泥形成期间的平均产气率为５．３Ｌ／Ｌ·ｄ，最高产气率达到了８．１Ｌ／Ｌ·ｄ，进水和出水ＣＯＤ分别为３０００～５５００ｍｇ／Ｌ和８００～３０００ｍｇ／Ｌ，平均ＨＲＴ为１０．４ｈ，平均出水乙酸、丙酸和丁酸浓度分别为２６４．１、５１９．８和１０４．６ｍｇ／Ｌ，所产气体的平均甲烷含量为６０．６％，颗粒污泥为黑色，直径０．５～３．０ｍｍ不等，沉降速率为１１９．６ｍ／ｈ，其中的优势产甲烷菌为鬃毛甲烷菌（Ｍｅｔｈａｎｏｓａｅｔａ），而甲烷八叠球菌（Ｍｅｔｈａｎｏｓａｒｃｉｎａ）存在甚少。The UASB reactor of lab scale was used to treat soybean cake wastewater in order to incubate anaerobic granular sludge. Gas production rate, influent and effluent COD, HRT, VFA concentration (acetate, propionate and butyrate) and mathane content were determented , and the predominant methanogens were observed by SEM and TEM to demonstrate the process and characteristics of anaerobic granular sludge. The results indicated that anaerobic granular sludge was formed during the performance of 50 days;average gas production rate was 5.3L/L·d;the highest gas production rate reached up to 8.1L/L.d, influent and effluent COD was 3000～5500mg/L and 800～3000mg/L respectively;average HRT was 10.4h;average concentration of effluent acetate, propionate and butyrate were 264.1mg/L, 519.8mg/L, and 104.6mg/L respectively;average methane content was 60.6%, granular sludge containing mainly Methanosaeta and less Methanosarcina was dark with diameter of 0.5～3.0mm and precipitation rate of 119.6m/h. 

关 键 词：UASB 厌氧颗粒污泥 甲烷菌 固定化 废水处理 

分 类 号：X703[环境科学与工程—环境工程] X520.5