左卡尼汀口服液体外抗氧化活性  

Antioxidafive Effects of L-carnitine Solution in Vitro

作　　者：梁孝印[1,2] 邵士川[1,2] 贺伟[3] 曹玉[4] 周茂金[2] 王春波[1] 

机构地区：[1]青岛大学医学院,山东青岛266001 [2]泰安市中心医院 [3]山东省泰山疗养院 [4]青岛大学附属医院

出　　处：《潍坊医学院学报》2009年第3期208-210,共3页Acta Academiae Medicinae Weifang

摘　　要：目的观察左卡尼汀体外清除自由基、抗氧化活性。方法超氧阴离子(O_2^-)由黄嘌呤(X)与黄嘌呤氧化酶(XOD)反应体系产生,羟自由基(·OH)由Fenton反应体系产生,观察不同浓度下左卡尼汀口服液产生超氧阴离子自由基(O_2^-)和羟自由基(·OH)的能力;测定H_2O_2诱导大鼠红细胞氧化溶血及对EDTANa_2-Fe^(2+)-H_2O_2系统所产羟自由基诱生的大鼠红细胞膜丙二醛(MDA)含量的影响来观察左卡尼汀的生物活性。结果分别在10～100g/L及1～100g/L浓度范围内,左卡尼汀口服液产生超氧阴离子自由基和羟自由基的能力随浓度降低而增加且与稀释倍数呈良好的线性关系,直线回归方程分别为Y=14.0652+24.9066X(O_2^-)与Y=34.2673+38.0382X(·OH),相关系数(r)分别为0.9830(O_2^-)和0.9969(·OH);在10～100g/L浓度范围内,显著抑制H_2O_2诱导的大鼠红细胞氧化性溶血并显著降低大鼠红细胞膜丙二醛含量,且抑制能力与浓度呈良好的线性关系,直线回归方程分别为Y=23.5309+0.293X与Y=-0.6978+0.8971X,r分别为0.9811和0.9998。结论左卡尼汀具清除自由基及抗脂质过氧化的作用。Objective To investigate the antioxidative effect of L-carnitine oral solution in vitro. Methods Superoxide anion （ O2^ - ） was produced by the X and XOD system and hydroxyl radical（ · OH ） was produced by Fenton reaction. Observing the influence of L- carnitine in different concentrations on the oxidative hemolysis of rats induced by hydrogen peroxide and the content of MDA in the membrane of rats＇hematid induced by hydroxyl radical produced in EDTANa2-Fe2 ^＋ - H2O2 system. Results The results indicate that the productivity of superoxide anion and hydroxyl radical respectively in the concentration of 10 - 100g/L （ O2^ - ） and 1 - 100g/L （ · OH ） increase with the decrease of concentration of L-earnitine solution and present fine linearity with dilution times. The linear regression equations respectively are Y = 14.0652 ＋ 24.9066X （ O2 ^- ） and Y = 34. 2673 ＋ 38. 0382X （ · OH ）. The coefficient of correlations individually are 0. 9830 （ O2^ - ） and 0.9969（ · OH） （P 〈 0.01 ）. L-carnitine solution can effectively restrain the oxidative hemolysis of rats and decrease the concentration of MDA in the membrane of rats＇hematid in the concentration of 10 - 100g/L. The linear regression equations respectively are Y = 23. 5309 ＋ 0. 293X and Y = -0. 6978 ＋ 0. 8971X. The coefficient of correlations respectively are 0.9811 and 0. 9998 （ P 〈 0.01 ）. Conclusion L-carnitine has the evident effect of eliminating reactive oxygen species and decreasing lipid peroxidation.

关 键 词：左卡尼汀 自由基 抗氧化作用 

分 类 号：R965[医药卫生—药理学]