咔唑法同时测定玻璃酸钠和硫酸软骨素的含量  

Carbazole method for simultaneous determination of sodium hyaluronate and chondroitin sulfate

作　　者：万秀玉 白若琬 姜雯[2] 张天民[3] 

机构地区：[1]山东博士伦福瑞达制药有限公司,山东济南250101 [2]辽宁省食品药品检验所,辽宁沈阳110023 [3]山东大学药学院,山东济南250012

出　　处：《药学实践杂志》2009年第4期282-283,293,共3页Journal of Pharmaceutical Practice

摘　　要：目的:建立咔唑法同时测定玻璃酸钠(SH)和硫酸软骨素(CS)含量的方法。方法:通过醋酸钠调节样品的酸碱度和离子强度,氯化十六烷基吡啶(CPC)可选择性地沉淀CS,而SH不沉淀,从而将SH和CS进行分离。结果:样品预处理条件为:精密量取SH和CS的混合溶液10 mL,加入醋酸钠1.6 g,12.5 mmol/LCPC10 mL,充分搅拌后,静置1 h,20μm滤膜过滤;滤液用于测定SH的含量;滤饼经1.4 mol/L氯化钠溶液超声解离后,用于测定CS的含量;SH和CS的平均回收率分别为101.2%和99.4%,RSD分别为1.05%和0.96%。结论:本法可同时测定SH和CS的含量,相互之间无干扰。Objective ：To establish a carbazole method for the simultaneous determination of sodium hyaluronate （SH） and chon- droitin sulfate （CS）. Methods： By regulating acidity or alkalinity and ionic strength with sodium acetate,CS was selectively precipita- ted with cetylpyridinium chloride （CPC） ,whereas SH was not. So SH and CS were separated. Results：The sample pretreatment condi- tions were as follows：Measuring 10 mL mixture of SH and CS accurately,adding 1.6 g sodium acetate and 10 mL CPC of 12.5 mmol/ L, standing for 1 h after stirring thoroughly, then, the solution was filtered with 20 μm filter membrane and the filtrate was used to deter- mine the content of SH ;the precipitates on the filter membrane were resolved with 1.4 mol/L NaC1 solution by ultrasonic treatment for the determination of CS. The average recoveries of SH and CS were 101.2% and 99.4% with RSD of 1.05% and 0.96% ,respectively. Conclusion ：This method can be used for the simultaneous determination of SH and CS without interference with each other.

关 键 词：咔唑法 玻璃酸钠 硫酸软骨素 氯化十六烷基吡啶 含量测定 

分 类 号：R94[医药卫生—药剂学]