HPLC法同时测定淫羊藿药材中的5个黄酮类成分  

Simultaneous determination of five flavone constituents in Epimedium Brevicornum by HPLC

作　　者：夏爱军[1] 黄河舟[2] 高岗[2] 张海[3] 朱臻宇[2] 柴逸峰[2] 

机构地区：[1]中国人民解放军第303医院,广西南宁530021 [2]第二军医大学药学院,上海200433 [3]第二军医大学东方肝胆外科医院,上海200438

出　　处：《药学实践杂志》2009年第4期284-285,288,共3页Journal of Pharmaceutical Practice

摘　　要：目的：建立同时测定淫羊藿药材中5种成分的HPLC方法。方法：采用Diamonsil—C18柱（4．6mm×250mm，5μm）；流动相：乙腈和甲酸水（pH=3．0），梯度洗脱；流速：0．9mL／min；DAD检测，检测波长：270nm。结果：淫羊藿药材中5个主要黄酮类成分朝藿定A、朝藿定c、淫羊藿苷、箭藿苷A、宝藿苷1分别在0．7800～83．20μg/mL、2．081～222．0μg／mL、3．288～350．7μg／mL、0．8311～88．65μg／mL、0．3518～37．52μg／mL的浓度范围内呈良好的线性关系，最低检测限分别为0．50、0．75、0．87、0．32、0．12μg／mL。重复性、稳定性实验结果的RSD〈3％；各成分平均回收率均小于107％，RSD〈6％。结论：该方法快速简便、准确可靠，各主要化学成分均能达到基线分离，适用于淫羊藿药材的质量控制。Objective： To establish a HPLC method for simuhaneous determination of five flavone constituents in Epimedium Brevi- cornum. Methods ：The determination was performed on Diamonsil-C18 column（4.6 mm× 250 mm,5 μm , Dikma）. The mobile phase was consisted of acetonitrile-fm＇mic acid aqueous solution with a flow rate of 0.9 mL/min. The detection wavelength was set at 270 nm. Results：A method has been established for determination of epimedin A~ , epimedin B, icariin, sagittatoside A, baohuoside I in one run by HPLC. Conclusion：The established method is simple, convenient, accurate and reliable, and suitable for the quality control of Epimedium Brevicornum.

关 键 词：朝藿定A 朝藿定C 淫羊藿苷 箭藿苷A 宝藿苷Ⅰ 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]