人参茎叶总皂苷中人参皂苷Re的含量分析  

Determination of ginsenoside R_e in total ginsenoside from stalk and leaf of Panax ginseng

作　　者：何厚罗[1] 黄章倍[1] 盛佳钰[1] 岳小强[1] 李敏[2] 辛海量[1,3] 

机构地区：[1]第二军医大学中医系,上海200433 [2]第二军医大学海医系军队卫生教研室,上海200433 [3]中国人民解放军第546医院,新疆乌鲁木齐841700

出　　处：《药学实践杂志》2009年第4期291-293,共3页Journal of Pharmaceutical Practice

基　　金：总后"十一五"专项项目(No.06Z024);"十一五"军队中医药研发推广专项课题(No.2006172002)

摘　　要：目的:分析人参茎叶总皂苷中人参皂苷Re的含量,为人参总皂苷的质量控制提供依据。方法:色谱柱:D iamonsil C18(4.6 mm×25 mm,5μm),保护柱:D IKMA EasyGuard C18(10 mm×4.6 mm),流动相:乙腈-0.5%冰醋酸水溶液,梯度洗脱,流速:1.25 mL/m in;ELSD:漂移管温度40℃,载气压力3.5 bar,放大系数为7。结果:标准曲线:C=1.191×10-7A-0.187 6,线性范围:0.038~1.14 mg/mL,r=0.999 3,检出限:20 ng(S/N>3),加样回收率:96.84%~104.21%。结论:该方法前处理简单,分析准确、快速,可作为人参茎叶总皂苷中人参皂苷Re的含量分析方法。Objective ： To determine quantitatively ginsenoside Re in total ginsenoside from stalk and leaf of Panax ginseng, and to provide experimental evident for total ginsenoside quality control of Panax ginseng. Methods ： The method was developed with the con- dition as follows ： Diamonsil C js （4.6 mm x 25 mm,5 p^m） and DIKMA EasyGuard C ls （10 mm ~ 4.6 mm） was used as chromatograph- ic column and guard column respectively. Gradient elution was employed with the mobile phase of acetonitrile--0.5% acetic acid/water at a flow rate of 1.25 mL/min. ELSD：temperature of the drift tube 40 ℃ ; gas pressure： 3.4 bar; gain： 7. Results：The standard curve ： C -- 1.191 × 10^ - 7A - 0. 187 6 ; the linear range ： 0. 038 - 1.14 mg/mL, r = 0. 999 3 ; the limits of detection ： 20 ng （ S/N 〉 3 ） ; recovery rate of the added sample： 96.84% - 104.21%. Conclusion：With its accuracy, sensitivity, reproducibility as well as the sim- plicity of sample preparation, this method is feasible for quality control of total ginsenoside from stalk and leaf of Panax ginseng.

关 键 词：总皂苷 人参皂苷RE HPLC-ELSD 

分 类 号：R917[医药卫生—药物分析学]