HPLC法测定仙乐雄胶囊中淫羊藿苷的含量  

Determination of icariine in xianlexiong capsules by HPLC

作　　者：程世云[1] 郝自新 

机构地区：[1]安徽省食品药品检验所,安徽合肥230051 [2]安徽省安庆市药品检验所,安徽安庆246001

出　　处：《安徽医药》2009年第8期899-900,共2页Anhui Medical and Pharmaceutical Journal

摘　　要：目的建立仙乐雄胶囊中淫羊藿苷的含量测定方法。方法采用高效液相色谱法测定。色谱柱：C18柱,流动相：乙腈-水（28∶72）,流速：1.0 ml.min-1,检测波长：270 nm。结果淫羊藿苷在0.104-1.040μg之间线性关系良好,r=0.9999;平均加样回收率为98.7%,（n=6,RSD=0.51%）。结论试验表明,该方法操作简单,分离效果好,灵敏度高,可以用于仙乐雄胶囊的质量控制。Aim To establish an HPLC method for the determination of icariin in xianlexiong capsules.Methods C18 column was used.The mobile phase was acetonitrile-water（28∶72）.The flow rate was 1.0 ml·min-1.The detection wavelength was 270 nm.Results The linear range was from 0.104 to 1.040 μg（r=0.9999）and the average recovey was 98.7%（n=6,RSD=0.51%）.Conclusion The method is simple and sensitive with good separation efficiency.It can be used for routine quality control of xianlexiong capsules.

关 键 词：仙乐雄胶囊 淫羊藿苷 含量测定 HPLC 

分 类 号：R286[医药卫生—中药学]