短波紫外线与交联淀粉碘联用法对血浆中病毒灭活的研究  

STUDY ON INACTIVATION OF VIRUS IN PLASMA BY COMBINATION OF ULTRAVIOLET (C) RAYS AND CROSSLINKED STARCH-IODINE

作　　者：熊鸿燕[1] 刘育京[1] 涂瀛 

机构地区：[1]第三军医大学预防医学系

出　　处：《中国消毒学杂志》1998年第3期134-149,共16页Chinese Journal of Disinfection

摘　　要：采用细胞感染试验、全自动生化分析仪、ＳＤＳ－ＰＡＧＥ等技术，观察短波紫外线（ＵＶＣ）与交联淀粉碘（ＣＳＩ）联用对血浆中辛德比斯病毒（ＳＶ）的灭活效果及血浆成分的变化。结果，以７１７０Ｊ／ｍ２ＵＶＣ照射后再经１００ｍｇ／ｍｌＣＳＩ处理６０ｍｉｎ，血浆中病毒的灭活可达６个对数级，且两消毒因子有协同增效作用。消毒后的血浆，除乳酸脱氢酶完全消失，总胆红素含量明显下降，钾离子含量比对照增加５２倍外，多种蛋白含量、蛋白分子量和生化指标均维持在正常值范围。he efficacy of combination of ultraviolet rays (UVC) and crosslinked-starch iodine (CSI) in inactivating Sindbis virus (SV) in plasma and changes in plasma components were observed using techniques such as cell infection test, automatic biochemical analysis and SDS-PAGEThe results indicated that the efficacy of the method using UVC irradiation preceding CSI was better than that of the method using CSI preceding UVC irradiation or using simultaneously CSI and UVC irradiation 7 170 J/m2UVC irradiation followed by 100 mg/ml CSI treatment for 60 min could inactivate the virus in plasma by up to 6 lg and the two virucidal agents showed good synergetic effect In the plasma after disinfection, except complete disappearance of lactic dehydrogenase, obvious decrease in content of total bilirubin and 52 fold increase in K+ content as compared with control, the content of a variety of proteins, the molecular weight and biochemical parameters of proteins were maintained at normal ranges

关 键 词：紫外线 交联淀粉碘 血浆 消毒 

分 类 号：R457.14[医药卫生—治疗学] R187[医药卫生—临床医学]