耐碳青霉烯类鲍曼不动杆菌金属β-内酰胺酶的研究  

Study of metallo-β-lactamase of Carbapenems-resistant Acinetobacter baumanii

作　　者：雷金娥[1] 袁莉[1] 李和平[1] 曾晓艳[1] 

机构地区：[1]西安交通大学医学院第一附属医院,陕西西安710061

出　　处：《现代中西医结合杂志》2009年第27期3280-3281,共2页Modern Journal of Integrated Traditional Chinese and Western Medicine

摘　　要：目的了解我院鲍曼不动杆菌产生金属β-内酰胺酶(MBLs)的情况。方法分别用金属螯合剂乙二胺四乙酸(EDTA)和2-巯基丙酸抑制试验测定鲍曼不动杆菌产金属β-内酰胺酶情况。结果用头孢他啶+2-巯基丙酸法检出产金属酶菌株52株(81.2%),亚胺培南+EDTA法检出45株(70.3%),2种方法均阳性36株(56.2%),头孢他啶+2-巯基丙酸法单独阳性16株(25.0%),亚胺培南+EDTA法单独阳性9株(14.1%),头孢他啶与EDTA有协同作用3株(4.7%)。97株耐亚胺培南鲍曼不动杆菌金属β-内酰胺酶的检出率为66.0%(64/97)。结论金属β-内酰胺酶的产生是鲍曼不动杆菌对碳青霉烯类抗生素耐药的重要机制之一,实验室应加强对产酶菌株的监测,合理使用抗生素。Objective It is to investigate the generation of metallo-β-lactamases （MBLs） in imipenem-resistant Acinetobacter baumanii isolates. Methods The production of MBLs was detected by inhibition test using metal-chelator EDTA and 2 - mercaptopropionic acid. Results Ceftazidime ＋ 2 - mercaptopropionic acid method detected metallo-β-lactamase 52 strains （81.2%）, imipenem ＋ EDTA method 45 strains （70.3%）, two methods simultaneously positive 36 strains （56.2%）, ceftazidime sodium ＋ 2 mercaptopropionic acid method single positive 16 strains （25%）, imipenem ＋ EDTA method 9 strains （14.1% ）, ceftazidime and EDTA method 3 strains （4.7 % ） ;97 strains imipenem-resistant Acinetobacter baumanii metallo-β-1actamase detection rate was 66.0 % （64/97） o Conclusion The production of MBLs is one of the important mechanisms of Acinetobacter baumanii to carbapenems. Correct detection in laboratory should be necessary.

关 键 词：鲍曼不动杆菌 金属Β-内酰胺酶 亚胺培南 

分 类 号：R117[医药卫生—公共卫生与预防医学]