甘草甜素对HepG2.2.15细胞株HBV感染的影响  

Effect of Glycyrrhizin on Heptitis B Virus Infection in HepG2.2.15 Cell Line

作　　者：李永伟[1] 郭云蔚[1] 李刚[1] 凌小强[1] 李桂侠[1] 

机构地区：[1]中山大学附属第三医院,广东广州510630

出　　处：《中国中医药信息杂志》2009年第9期31-33,共3页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：教育部新世纪优秀人才支持计划(NCET-04-0797)

摘　　要：目的探讨甘草甜素(GL)对HepG2.2.15细胞上清液中HBsAg、HBeAg、HBV DNA的作用,及对细胞存活率的影响。方法实时荧光定量PCR检测HBV DNA的表达,ELASA检测HBV所分泌抗原,MTT检测细胞的增殖活性,计算细胞存活率。结果给药后各组HBsAg分泌结果显示总体均数间差异显著(P=0.000),GL各组与对照组比较差异有统计学意义(P<0.01),抑制作用存在剂量依赖关系;而对HBeAg水平的影响因剂量不同而表现为升高和降低两种趋势,除50μg/mL组外,其余各组与对照组比较,差异均有统计学意义(P<0.05),但800μg/mL组HBeAg分泌增加,400μg/mL以下组为HBeAg降低;HBV DNA的表达显示GL各组与对照组比较,差异均有统计学意义(P<0.05),但400μg/mL以下组为HBV DNA降低,800μg/mL组出现HBV DNA复制增强;MTT实验显示,200μg/mL以下3个剂量组均可促进细胞增殖(P<0.01),400、800μg/mL2组均显著抑制细胞增殖(P<0.01);MTT分别与HBeAg和HBV DNA水平呈显著负相关(P<0.01),但与HBsAg呈显著正相关(r=0.869,P=0.000)。结论GL对HepG2.2.15细胞株上清中的HBeAg和HBV DNA水平可能具有双向作用,而对HBsAg具有剂量依赖的抑制作用,提示GL的使用应注意选择适应证及合适的剂量。Objective To investigate the effect of glycyrrhizin （GL） on the expression of hepatitis B virus e antigen （HBeAg）, hepatitis B virus surface antigen （HBsAg）, HBV DNA levels and cell proliferation in HepG2.2.15 cell line. Methods HBV DNA level was examined by Real-time PCR. HBsAg and HBeAg levels were examined by ELASA, and cell proliferation was examined by MTT before and after stimulated with GL The GL groups were compared with the blank control group. Results HBsAg levels in the GL groups were inhibited in dose-dependent manner compared with the blank control group （P〈0.01）. HBeAg levels in GL 400, 200, 100 μg/mL groups were inhibited significantly compared with the blank control group （P〈0.05）. HBV DNA levels in GL groups with the dose below 400μg/mL were inhibited significantly compared with the blank control group. HBeAg and HBV DNA levels in the 800 μg/mL group were increased significantly compared with the blank control group （P〈0.05）. GL in the three groups of 200, 100, 50 μg/mL could significantly promote cell proliferation, respectively （P〈0.05}. The 400 μg/mL and 800 μg/mL groups inhibited the growth of cells significantly （P〈0.01）. Cell proliferation were notably negative correlated with the expression of HBeAg and HBV DNA levels （P〈0.05）, but positive correlated with HBsAg levels （r =0.869, P =0.000）. Conclusions GL could inhibit or promote cell proliferation, HBeAg level and HBV DNA level in HepG2.2.15 cell line, while inhibit HBsAg secretion in dose-dependent manner. To choose appropriate disease and dose with GL treatment is important.

关 键 词：甘草甜素 乙型肝炎病毒 HepG2.2.1 5细胞株 

分 类 号：R285.5[医药卫生—中药学]