中药补骨脂素联合长波紫外线诱导K_(562)、NB_4细胞凋亡时对线粒体膜电位的影响  

Effects of Psoralen Combined with UVA on Mitochondrial Membrane Potential during Apoptosis in K_(562) and NB_4 Cells

作　　者：吴晓华[1] 周思维[2] 陈楠楠[1] 向阳[1] 张晨[1] 黄世林[1] 

机构地区：[1]解放军第210医院,大连116021 [2]大连大学附属新华医院,大连116021

出　　处：《中国中医急症》2009年第9期1481-1482,共2页Journal of Emergency in Traditional Chinese Medicine

基　　金：国家自然科学基金项目(No.30472257)

摘　　要：目的研究中药补骨脂素(PSO)联合长波紫外线(UVA)光化学疗法(PUVA)诱导人白血病细胞K562、NB4凋亡时对线粒体膜电位(ΔΨm)的影响。方法细胞与不同质量浓度PSO,接受或不接受UVA照射后共同培养,电镜下观察细胞超微结构改变,流式细胞仪(FCM)检测细胞凋亡率和线粒体膜电位变化,采用多因素方差分析法进行统计学处理。结果PUVA处理后的K562、NB4细胞超微结构出现明显的凋亡形态学改变;PSO、UVA照射及PUVA均可使细胞凋亡率增加,线粒体膜电位下降,PUVA的作用显著强于前两者。结论PUVA可诱导人白血病细胞发生凋亡,诱导凋亡的途径之一为下调线粒体膜电位水平。Objective： To study the effects of psoralen （PSO） combined with photochemotherapy of UVA （PUVA） on mitochondrial membrane potential in K562 and NB4 cells. Methods： Cells were incubated with PSO in different mass concentrations irradiated with or without UVA. The changes of uhrastructure of cells were observed through the electron microscope. The apoptosis ratios and the changes of mitochondrial membrane potential were detected through the flow cytometry. The factorial design and analysis of variance were used to analyze the interaction among the factors. Results： There were obvious uhrastructure changes about apoptosis in K562 and NB4 cells after being treated with PUVA. PSO, UVA and PUVA all increased the apoptosis ratios and decreased the mitochondrial membrane potential, and the effects of PUVA were the strongest. Conclusion： PUVA can induce the apoptosis of K562 and NB4 cells and one of the pathway to induce apoptosis is to downregulate the mitochondrial membrane potential.

关 键 词：PUVA 白血病 K562细胞 NB4细胞 凋亡 线粒体膜电位 

分 类 号：R285.5[医药卫生—中药学]