急性心肌缺血再灌注家兔白细胞变形能力变化及654-2对其影响  

THE CHANGE OF LEUKOCYTE DEFORMABILITY IN RABBIT AFTER ACUTE MYOCARDIAL ISCHEMIA REPERFUSION AND EFFECT OF 654-2 ON IT

作　　者：于春华[1,2,3,4] 许开利[1,2,3,4] 刘宗宝[1,2,3,4] 谭秀彦 韩龙涛 刘成玉 

机构地区：[1]青岛医学院第二附属医院药剂科 [2]青岛医学院松山医院检验科 [3]青岛卫生学校 [4]青岛医学院诊断学教研室

出　　处：《青岛医学院学报》1998年第3期188-190,共3页Acta Academiae Medicinae Qingdao Universitatis

摘　　要：目的探讨缺血再灌注家兔白细胞变形能力（ＬＤ）的变化及６５４－２对其影响。②方法采用结扎／放开冠状动脉左旋支造成家兔急性心肌缺血再灌注模型，采用ＤＸＣ－３００Ａ型核孔膜红细胞变形能力测定仪检测了ＬＤ和血浆总超氧化物歧化酶（ＳＯＤ）活力的变化。③结果缺血再灌注组家兔ＬＤ与ＳＯＤ均明显降低，与对照组、缺血组比较，差异有极显著性（Ｆ＝２１．７６，１８．９２，ｑ＝８．５２～１７．０８，Ｐ＜０．０１）。应用６５４－２治疗后，ＬＤ与ＳＯＤ均升高，与缺血再灌注组比较，差异有极显著性（ｑ＝９．２６，８．７６，Ｐ＜０．０１）；与对照组比较差异无显著性（ｑ＝１．５２，２．０６，Ｐ＞０．０５）。缺血再灌注组、缺血组和６５４－２组家兔ＬＤ与ＳＯＤ呈正相关（ｒ＝０．８２３～０．８８４，Ｐ＜０．０１）。④结论急性心肌缺血再灌注家兔ＬＤ明显降低。Objective To explore the change of leukocyte deformability (LD) in rabbit after acute myocardial ischemia reperfusion and effects of 654-2 on it. Methods The acute ischemia/reperfusion model was made by tying and loosing the left bundel of coronary artery of rabbit, and the LD was measured by using DXC300A erythrocyte deformability apparatus and plasma SOD was measured with spectrophotometer. Results LD and SOD in ischemia/reperfusion group were significantly lower than those of control and ischemia groups(F=21.76,18.92,q=8.52—17.08,P<0.01).　After treatment with 654-2,the LD and SOD were higher in 6542 group than those in ischemia/reperfusion group (q=9.26,8.76,P<0.01),　but there was no differece between 654-2 group and controls (q=1.52,2.06,P>0.05).In ischemia/reperfusion, ischemia and 6542 group, LD was positively related to SOD (r=0.823—0.884,P<0.01). Conclusion LD was significantly lower in acute ischemia/reperfusion group, and 654-2 might enhance LD.

关 键 词：心肌缺血 白细胞 变形能力 再灌注损伤 654-2 

分 类 号：R542.2[医药卫生—心血管疾病] R972[医药卫生—内科学]