水稻显性早熟基因Ehd的SSR标记定位  

Mapping of the Dominant Earliness Gene Ehd Using SSR Markers in Rice

作　　者：朱文银[1,2] 李文涛[1] 丁效华[1] 张泽民[1] 曾瑞珍[1] 朱海涛[1] 张桂权[1] 

机构地区：[1]华南农业大学广东省植物分子育种重点实验室,广东广州510642 [2]山东省水稻研究所,山东济宁272177

出　　处：《华北农学报》2009年第4期7-10,共4页Acta Agriculturae Boreali-Sinica

基　　金：国家自然科学基金(30600390)

摘　　要：以籼稻品种广陆矮4和粳稻品种台中65为亲本构建高世代回交分离群体,选用分布于水稻全基因组的145个SSR标记对亲本及抽穗期早熟基因进行分析。结果表明,114个标记在亲本间具有多态性,多态率78.6%;在BC3F1群体中,检测到10个标记的基因型来源于供体亲本广陆矮4号;在BC3F2定位群体中,早熟植株数与晚熟植株数的分离比例为3∶1,早熟植株平均比晚熟植株提早抽穗21 d;通过SSR标记与抽穗期共分离分析将显性早熟基因Ehd界定在分子标记RM271和RM258之间;Ehd与标记RM184和RM271紧密连锁,遗传距离分别为2.6 cM和2.1 cM,此结果为该基因分子标记辅助选择奠定了基础。In the paper, an indica variety Guangluai 4 and a japonica variety Taichung 65 were used to construct high generational backcross segregation population. Using 145 SSR markers which distributed on rice chromosomes, the polymorphism of parents and heading date of a BC3F2 mapping population were analyzed. The results showed that 114 markers displayed polymorphism between two parents, the rate of polymorphism is 78.6 % ; In BC3F1, the genetic background analysis detected ten SSR markers, which distributed on rice chromosome 2,3,4,7 and chromosome 10;In BC3F2 mapping population, the early-heading plants and late-heading plants segregated in a ratio of 146：47, fitting the expected ratio of 3 ： 1, the former was 21 days earlier than the latter; Linkage analyses suggested that the gene Ehd was tightly linked with SSR marker RM184 and RM271 ,the genetic distance between the two markers and Ehd were 2.6 eM and 2.1 cM, respectively. The mapping of the locus Ehd would be useful for its molecular marker-assisted selection.

关 键 词：水稻 SSR标记 抽穗期基因 分子标记辅助选择 

分 类 号：Q78[生物学—分子生物学]