ATP是构建类似C_4水稻的重要限制因素  

A Key Limited Factor ATP of Constructing C_4-like Rice

作　　者：张边江[1] 凌丽俐[2] 陈全战[1] 华春[1] 焦德茂[3] 

机构地区：[1]南京晓庄学院生命科学系,江苏南京211171 [2]西南大学柑橘研究所,重庆400712 [3]江苏省农业科学院,江苏南京210014

出　　处：《华北农学报》2009年第4期17-22,共6页Acta Agriculturae Boreali-Sinica

基　　金：国家转基因专项(16100004);江苏省教育厅高校自然基金项目(08KJD180012);南京晓庄学院生态学重点学科(2005NXY01)

摘　　要：为了分析ATP是否是转PEPC+PPDK基因水稻或转PEPC+PPDK+ME基因水稻CO2浓缩过程的限制因素,以原种和不同转C4光合酶基因水稻为材料,测定了C4光合酶活性、光合速率以及活性氧代谢有关指标,结果表明:原种中具有全套的C4光合酶,但活性很低,而不同转C4光合酶基因水稻高表达了相应的C4酶活性。在高光条件下,与原种相比较,转PPDK基因水稻的光合速率未增加;转ME基因水稻的光合速率降低了6.1%;转PEPC+PPDK双基因水稻与转PEPC基因水稻相近。ATP和ATP激活剂NaHSO3处理后,可显著提高转PEPC+PPDK双基因水稻和转PEPC+PPDK+ME基因水稻的光合放氧速率,达到玉米的80.7%,显现出类似C4的光合特点,表明ATP是构建类似C4水稻的重要限制因素。光氧化条件下,转PEPC+PPDK+ME基因水稻的耐光氧化能力得到进一步的增强。这些结果为构建C4水稻提供了技术途径。With untransformed rice and transgenic rices with different C4 photosynthesis genes as the materials, the activity of C4 photosynthesis enzymes, the net photosynthetic rate （Pn）and the indexes of active oxygen metabolism were determined. The activities of C4 photosynthesis enzymes in untransformed rice were very low, while those of corresponding enzymes in different transgenic rices were all highly expressed. The Pn of the PPDK transgenic rice was not increased, that of the ME transgenic rice was decreased by 6.1%, that of the transgenic rice with PEPC and PPDK genes（ CK）was similar with that of the PEPC transgenic rice under high light intensity. After treatment with ATP and its activator NaHSO3, the photosynthetic rate of O2 evolution were significantly increased in the CK transgenic rice and the transgenic rice with PEPC, PPDK and ME genes（CKM）, which reached 80.7 % of that in maize and exhibited photosynthetic character of C4- like plants. This indicated that ATP is the key limited factor for constructing C4-1ike rice. In addition, after MV（ artificial photooxidative activator）treatment, the CKM transgenic rice was more tolerant to photooxidative stress. These results would provide a new technical approach to constructing C4-like rice.

关 键 词：转基因水稻 光合特性 C4光合循环 三磷酸腺苷(ATP) 

分 类 号：Q78[生物学—分子生物学]