牛肌肉生长抑制素基因编码区全序列的分子进化特征  

Molecular Evolution Character of Complete Coding Sequence of Bovine Myostatin Gene

作　　者：王兰萍[1] 耿荣庆[1] 冀德君[2] 常洪[2] 常春芳[2] 李永红[2] 

机构地区：[1]盐城师范学院生命科学与技术学院,江苏盐城224051 [2]扬州大学动物科学与技术学院,江苏扬州225009

出　　处：《华北农学报》2009年第4期46-49,共4页Acta Agriculturae Boreali-Sinica

基　　金：江苏省高校自然科学基础研究项目(08KJB230002);江苏省高校"青蓝工程";国家自然科学基金项目(30571323)

摘　　要：运用PCR分段扩增测序法,获得普通牛、瘤牛、牦牛、大额牛、水牛（沼泽型和河流型）MSTN基因编码区全序列,进而分析编码区序列的分子进化特征。结果显示,牛MSTN基因编码区全序列长1128 bp,种内核苷酸突变率为0-0.35%,种间核苷酸突变率为0.08%-1.42%。MSTN基因编码区序列存在密码子使用偏好性,29个偏好性密码子约占密码子使用总数的49.2%。核苷酸的替代以转换为主,转换/颠换比为2.9。非同义突变位点远少于同义突变位点,同义与非同义替代速率比全部小于或等于1,表明MSTN基因编码区序列并未受到达尔文正向选择的影响,却受到一定的负向选择作用。分子进化树显示,水牛、瘤牛独自成类并与其他牛种显著分化;普通牛、牦牛、大额牛间的序列分化程度并不明显,但有可能在进化过程中逐步产生明显的分化。We obtained complete coding sequence of myostatin gene from Bos taurus, Bos indicus, Bos grunniens, Bos frontalis and Bubalus bubalis （swamp buffalo and river buffalo）by PCR fragments sequencing method. The molecular evolution characters of CDS of myostatin gene were analyzed. The result showed that CDS of bovine MSTN gene was 1 128 bp in length. The mutation rate within species was between 0 and 0.35 %, while the mutation rate between species was between 0.08 % and 1.42%. The codon usage bias phenomenon arosed and the 29 preferenced codon possessed about 49.2 % of the total preferenced codons. The predominant substitution model of nucleotide was transition and the ratio of transition and transversion was 2.9. Nonsynonymous sites were far less than synonymous sites. The ratio of synonymous and nonsynonymous substitution were all less or equal to one manifesting that it did not effected by Darwin positive selection. But some sites affected by negative selection. Molecular tree showed that haplotypes from Bubalus bubalis and Bos indicus clustered on their own and there were apparent differentiation between Bubalus bubalis, Bos indicus and other bovine species. There was no apparent differentiation among Bos taurus, Bos grunniens, Bosfrontalis and they maybe produced apparent differentiation gradually in the process of evolution.

关 键 词：牛种 肌肉生长抑制素基因 编码区序列 分子进化 

分 类 号：Q78[生物学—分子生物学]