低温锻炼对低温胁迫下菊花生理活性的影响  

Effects of Cold Acclimation on Physiological Metabolism of Chrysanthemum under Low Temperature Stress

作　　者：李云[1,2] 杨际双[1] 张钢[1] 陈段芬[1] 

机构地区：[1]河北农业大学园艺学院,河北保定071001 [2]青岛市现代农业示范园,山东青岛266112

出　　处：《华北农学报》2009年第4期179-182,共4页Acta Agriculturae Boreali-Sinica

基　　金：河北农业大学留学回国人员科研启动基金项目(2004-897);教育部留学回国人员科研启动基金项目(2006-1018)

摘　　要：为揭示低温锻炼下菊花生理变化特点及特异性表现,以切花菊(Chrysanthemum morifolium)秋白菊叶片为试验试材,在4℃下对其进行5 h的低温锻炼,然后在-5℃下分别进行0,0.5,1,3和5 h不同时间的低温胁迫。通过对其相对电导率、丙二醛(MDA)和脯氨酸含量及5′-核苷酸酶、过氧化物歧化酶(POD)和超氧化物歧化酶(SOD)活性的测定,研究了低温锻炼对菊花抗寒性的诱导。结果表明,低温锻炼的菊花叶片相对电导率及MDA含量比对照减小,而脯氨酸含量、SOD和5′-核苷酸酶活性比对照增加,说明低温锻炼在一定程度上提高了菊花植株的抗寒性。To investigate the effects of cold acclimation on the cold tolerance, the leaves of chrysanthemum （ Chrysanthemum morifolium） were treated at 4 ℃ for 5 hours, and then the changes of electrolytic leakage, content of malondialdehyde level （MDA）, soluble protein, proline and activity of 5＇-nucleotidase, superoxide dismutase （SOD）and peroxidase （POD）were measured after low temperature stress with different times （0.5- 5 h）.The results showed that the electrolytic leakage and the content of MDA in leaves treated with cold acclimation were lower than those of non-treated, the contents of proline and the activities of SOD and 5＇-nucleotidase kept higher levels compared with those of controls. The results indicated that the cold tolerance of chrysanthemum was improved by cold acclimation treatment.

关 键 词：菊花 低温锻炼 低温胁迫 抗寒性 

分 类 号：S682.1[农业科学—观赏园艺]