含BDM及钙心脏保存液保存离体鼠心  

The Preservation of the Isolated Rat Hearts with 2,3-Butadione Monoxime and Calcium(BDM)

作　　者：王敏敏[1] 吴熹[1] 马旺扣[1] 

机构地区：[1]南京铁道医学院附属医院胸心外科

出　　处：《铁道医学》1998年第4期220-222,共3页Railway Medical Journal

摘　　要：目的研究含二乙酰—肟（２，３－ｂｕｔａｎｅｄｉｏｎｅｍｏｎｏｘｉｍｅ，ＢＤＭ）及Ｃａ２＋的Ｅｕｒｏ－Ｃｏｌｉｎｓ晶体保存液对离体鼠心脏的保存作用。方法采用离体工作心模型，观察保存前后鼠心的心功能、心率、肌酸磷酸激酶释放及超微结构的变化。结果含ＢＤＭ及Ｃａ２＋保存液保存的鼠心其左心室收缩压（ＬＶＳＰ），左室压力变化最大速率（ＬＶ±ｄｐ／ｄｔｍａｘ）及心率（ＨＲ）与对照组无显著性差异，肌酸磷酸激酶释放及超微结构也显示细胞损伤程度轻于普通保存组。结论ＢＤＭ及Ｃａ２＋可增加心脏冠脉流量，改善心脏功能恢复。Objective To investigate the effectiveness of BDM and Ca^(2+) preserving cardiac function of hypothermic stored hearts.Method The isolated hearts were perfused in a storage buffer solution (Euro-Collins solution),hearts function,including left ventricular systolic pressure(LVSP),heart rate and coronary flow were examined during 1hr of normothermic reperfusion,the creatine kinase (CK) release and ultrastructure were also examined after experiment.Reaults Herats that had been perfused with BDM and Ca^(*2+) during hypothermic storage attained LVSP,and HR were not significantly different from values in the control group,CK and ultrastructure change of this group were significantly lower and better than that of the drug-free group.Conclusion BDM,and to a less extent Ca^(2+),applied improved cardiac function and decreased the damage to the cells.

关 键 词：二乙酰肟 钙 心脏保存 低温灌注 保存液 

分 类 号：R654.2[医药卫生—外科学] R318.52[医药卫生—临床医学]